相关实验视频

Updated: Sep 18, 2025

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

Published on: March 7, 2019

smc-5 的功能分离等位基因揭示了特定域的缺陷和对基因组维护至关重要的保存残留物

Haiyan Yuan^1,2, Arome Solomon Odiba^2,3, Guiyan Liao²

¹Department of Toxicology, School of Public Health, Guangxi Medical University, Nanning 530021, China.

|June 26, 2025

概括

该SMC-5/6复合体对于基因组稳定性至关重要. 新的Caenorhabditis elegans smc-5突变揭示了特定域如何影响DNA修复和复杂完整性,为研究基因组维护提供了工具.

科学领域:

分子生物学分子生物学
遗传学是一个遗传学.
细胞生物学细胞生物学

背景情况:

该SMC-5/6复合体对于保持基因组稳定性和DNA修复至关重要.
NSE-1是SMC-5/6复合体的重要组成部分,但其在Caenorhabditis elegans中的功能尚不清楚.
了解NSE-1的作用需要在SMC-5/6复合体的背景下进行详细的遗传和功能分析.

研究的目的:

为了识别和描述具有NSE-1表达或核定位缺陷的Caenorhabditis elegans突变物.
阐明SMC-5蛋白内特定域和保存残留物的功能意义.
为了产生新的遗传工具来剖析SMC-5/6复合体的体内功能.

主要方法:

通过使用NSE-1::GFP记者菌株进行了基于EMS的先进基因选.
使用SNP映射和全基因组测序,分离和映射了新的smc-5等位基因 (smc-5(wsh31),smc-5(wsh32),smc-5(wsh33).
进行了表型特征鉴定,包括幼大小,后代活力,雄性百分比,NSE-1::GFP局部化试验,以及对DNA破坏性物质 (MMS,HU,西斯普拉丁) 的过敏性测试.

主要成果:

确定了三种新的smc-5基因基因,包括两种切断和一种误解变异,影响SMC域中高度保存的残留物.
在所有突变体中观察到NSE-1::GFP核定位受损,截断导致完全损失,误解等位基因导致部分,依赖阶段的误定位.
已证明对DNA破坏性物质的异位基因特异性和发育阶段依赖的过敏性,表明功能分离的表型.

结论:

在SMC-5中,特定的域和保存的残留物对SMC-5/6复合体的完整性及其在基因组维护中的作用至关重要.
已识别的smc-5等位基因为进一步调查该复合体在DNA修复和基因组稳定性方面的功能提供了有价值的工具.
这些发现突出了在发育过程中SMC-5/6复合体内蛋白质结构,定位和功能之间的复杂关系.

关键词:

凯诺哈比迪斯 (Caenorhabditis elegans) 是一个可怕的生物.在NSE-1的本地化.复杂的SMC-5/6复合体基因组稳定性的基因组稳定性在SMC-5中,

更多相关视频

Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast

Author Spotlight: Enhanced Isolation of Interaction-Null Mutants in Yeast

Published on: December 29, 2023

CD Spectroscopy to Study DNA-Protein Interactions

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

相关概念视频

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Mismatch Repair

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...

Cohesins

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...

Restarting Stalled Replication Forks

Restarting Stalled Replication Forks

DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...

Nucleosome Remodeling

Nucleosome Remodeling

Nucleosomes are the basic units of chromatin compaction. Each nucleosome consists of the DNA bound tightly around a histone core, which makes the DNA inaccessible to DNA binding proteins such as DNA polymerase and RNA polymerase. Hence, the fundamental problem is to ensure access to DNA when appropriate, despite the compact and protective chromatin structure.
Nucleosome remodeling complex
Eukaryotic cells have specialized enzymes called ATP-dependent nucleosome remodeling enzymes. These enzymes...

Condensins

Condensins

Condensins are large protein complexes that use ATP to fuel the assembly of chromosomes during mitosis. They transform the tangled, shapeless mass of post-interphase DNA into individualized chromosomes by compacting, organizing, and segregating chromosomal DNA.
The plant and animal cells contain two types of condensin complexes—condensin I and condensin II. Both complexes have five subunits: two SMC (Structural Maintenance of Chromosomes) subunits, a kleisin subunit, and two HEAT-repeat...