银纳米粒子的功能依赖性细胞毒性:对赫西丁和美特罗尼达结合物的比较研究
Karol P Steckiewicz1,2, Monika Dmochowska1, Elżbieta Megiel3
1Department of Pharmaceutical Pathophysiology, Faculty of Pharmacy, Medical University of Gdansk, 80-211 Gdansk, Poland.
Biomolecules
|June 26, 2025
概括
银纳米粒子 (AgNPs) 的表面化学决定了它们的生物效应. AgNPs-CHL通过-银键诱导亡,而AgNPs-PEG-MET通过硫-银键触发了亡,影响了纳米粒子治疗设计.
科学领域:
- 纳米医学是一种纳米医学.
- 材料科学 材料科学 材料科学
- 毒理学 毒理学 毒理学
背景情况:
- 银纳米粒子 (AgNPs) 是有希望的治疗牙周炎.
- AgNP与药物的表面功能化影响其行为和有效性.
- 了解AgNP细胞毒性机制对于开发安全疗法至关重要.
研究的目的:
- 为了研究两个AgNP配方的细胞毒性:AgNPs-CHL和AgNPs-PEG-MET.
- 为了将表面化学 (-银与硫-银键) 与观察到的细胞毒性机制相关联.
- 评估表面功能化对AgNP物理化学性质和生物效应的影响.
主要方法:
- 使用UV-Vis,DLS和SEM-EDX进行物理化学表征.
- 对人类胎儿骨质母细胞 (hFOB 1.19) 和牙纤维母细胞 (HGF-1) 的细胞毒性评估.
- 试验包括BrdU,LDH,ROS检测,细胞周期分析,细胞亡试验和蛋白质表达.
主要成果:
- 在培养基中聚合AgNPs-CHL,而AgNPs-PEG-MET保持稳定.
- 两种AgNP都显示出度依赖的细胞毒性.
- AgNPs-CHL诱导的亡 (ROS,LDH释放,RIP1 / 3,MLKL),而AgNPs-PEG-MET诱导的亡 (卡斯巴-2). 这种亡是由于AgNPs-CHL的作用导致的.
结论:
- 表面功能化的类型显著影响AgNP的物理化学性质和生物效应.
- 在AgNPs-CHL中和银的相互作用导致了亡.
- 在AgNPs-PEG-MET中的硫-银键触发了亡,突出了针对治疗应用的定制设计.
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