Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Long-patch Base Excision Repair01:02

Long-patch Base Excision Repair

7.2K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.2K
Base Excision Repair01:54

Base Excision Repair

23.0K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
23.0K
Base-pairing and DNA Repair02:27

Base-pairing and DNA Repair

65.2K
65.2K
Nucleotide Excision Repair01:08

Nucleotide Excision Repair

37.6K
Overview
37.6K
Homologous Recombination02:31

Homologous Recombination

52.7K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
52.7K
Mismatch Repair01:20

Mismatch Repair

5.2K
Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
The Mutator Protein Family Plays a Key Role in DNA Mismatch Repair
The human genome has more than 3 billion base pairs of DNA per cell. Prior to cell division, that vast amount of genetic...
5.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Biochemical, structural and mutational landscapes of base excision repair enzymes and cancer: from atomic resolution to tumor signatures.

International journal of radiation biology·2026
Same author

The S180R Human Germline Variant of DNA Polymerase β Is a Low Fidelity Enzyme with Reduced Flexibility of the Fingers Domain.

Biochemistry·2026
Same author

Cell death pathways in response to <i>Mycobacterium tuberculosis</i> and other mycobacterial infections.

Infection and immunity·2025
Same author

Global screening of base excision repair in nucleosome core particles.

DNA repair·2024
Same author

Collapsed State Mediates the Low Fidelity of the DNA Polymerase β I260 Mutant.

Biochemistry·2024
Same author

Modifying the Basicity of the dNTP Leaving Group Modulates Precatalytic Conformational Changes of DNA Polymerase β.

Biochemistry·2024

相关实验视频

Updated: Sep 18, 2025

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.4K

蛋白质与蛋白质之间的相互作用在基切割修复中的作用.

Govardhan Rathnaiah1, Joann B Sweasy1

  • 1Fred and Pamela Buffett Cancer Center and Eppley Institute for Cancer Research, Omaha, NE 68198, USA.

Biomolecules
|June 26, 2025
PubMed
概括

本综述详细介绍了切除修复 (BER) 途径中的蛋白-蛋白相互作用,重点关注单核酸BER (SN-BER). 了解这些分子相互作用对于DNA修复洞察至关重要.

科学领域:

  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.
  • 生物化学 生物化学

背景情况:

  • 基因切割修复 (BER) 途径通过修复受损的DNA基因来维持基因组稳定性至关重要.
  • BER涉及一个复杂的蛋白质-蛋白质相互作用网络,促进有效的DNA修复.
  • 在BER中的关键酶包括DNA糖化酶,APE1,PNKP,Polβ,LigIIIα,PARP1/2和XRCC1.

研究的目的:

  • 审查单核酸BER (SN-BER) 途径中的蛋白质-蛋白质相互作用.
  • 探索SN-BER组件和其他调节蛋白之间的相互作用.
  • 提供关于BER路径协调机制的见解.

主要方法:

  • 文献综述,重点关注已发表的关于BER通路蛋白相互作用的研究.
  • 在SN-BER中分析蛋白质-蛋白质相互作用网络.
  • 基于证据的功能性讨论涉及SN-BER组件的相互作用.

主要成果:

  • 详细检查核心SN-BER酶之间的蛋白质与蛋白质相互作用.
  • 识别与SN-BER组件相互作用的调节蛋白.
  • 强调这些相互作用对于无的DNA中间交换的重要性.
关键词:
在APE1中,APE1是APE1.基因糖化酶是DNA的糖化酶.DNA聚合酶是一种DNA聚合酶.在XRCC1中,它是XRCC1.结合酶是一种结合酶.聚 (ADP-ribose) 聚合酶的聚合物

更多相关视频

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

9.7K
A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
11:58

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

Published on: March 5, 2018

8.4K

相关实验视频

Last Updated: Sep 18, 2025

Visualization of DNA Repair Proteins Interaction by Immunofluorescence
07:55

Visualization of DNA Repair Proteins Interaction by Immunofluorescence

Published on: June 26, 2020

10.4K
Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair
10:59

Atomic Force Microscopy Investigations of DNA Lesion Recognition in Nucleotide Excision Repair

Published on: May 24, 2017

9.7K
A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells
11:58

A Simple, Rapid, and Quantitative Assay to Measure Repair of DNA-protein Crosslinks on Plasmids Transfected into Mammalian Cells

Published on: March 5, 2018

8.4K

结论:

  • 蛋白质与蛋白质之间的相互作用对BER通路的效率和调节至关重要.
  • 了解这些相互作用为DNA修复机制提供了关键的见解.
  • 对这些相互作用的进一步研究可以揭示与DNA修复缺陷相关的疾病的新型治疗点.