双相CAPA-IVM改善了马的卵子质量和随后的胚胎发育,而不会引起遗传异常
Muhammad Fakhar-I-Adil1, Daniel Angel-Velez2, Emin Araftpoor3,4
1Ghent-Fertility and Stem Cell Team (G-FaST), Department for Reproductive Medicine, Department of Human Structure and Repair, Ghent University Hospital, Corneel Heymanslaan 10, 9000 Ghent, Belgium.
International journal of molecular sciences
|June 26, 2025
概括
与标准方法相比,试管成熟能力 (CAPA-IVM) 改善了马类卵细胞成熟和胚胎发育. 简短的CAPA-IVM显示了增强的潜力,遗传分析显示了由此产生的胚胎囊中高的euploidy率.
科学领域:
- 生殖生物学 生殖生物学
- 在体外受精过程中受精.
- 卵细胞成熟的过程
背景情况:
- 卵细胞的体外成熟 (IVM) 对具有对激素刺激的禁忌的患者至关重要.
- 与体内成熟的卵子相比,当前的IVM技术产生具有低于最佳发育能力的卵子.
- 容量IVM (CAPA-IVM) 使用C型尿素 (CNP) 的预成熟培养来增强卵细胞同步和能力.
研究的目的:
- 评估CAPA-IVM在改善马匹卵细胞质量和发育能力方面的有效性.
- 为了比较短 (6小时) 和长 (24小时) 的CAPA-IVM协议与标准IVM的结果.
主要方法:
- 使用标准IVM或CAPA-IVM (6小时或24小时预成熟) 成熟未成熟的马体积卵细胞复合体 (COC).
- 成熟的卵细胞被评估为释放的潜力,接受单卵细胞蛋白质组,并通过细胞内质精子注射 (ICSI) 用于胚胎发育.
- 发育的胚胎囊经过了染色体异常的遗传分析.
主要成果:
- 与对照组相比,CAPA-IVM显著增加了马类卵细胞成熟率.
- 简短的CAPA-IVM (6h) 显示出优越的胚胎发育潜力 (20/69) 与标准IVM (9/63) 相比.
- 蛋白质组学揭示了类似的景观,但在短CAPA-IVM卵细胞中发现了与细胞骨和核糖体功能相关的丰富基因组;在所有胚胎细胞中观察到高的euploidy率.
结论:
- CAPA-IVM对提高马匹卵细胞质量和发育能力有前途.
- 进一步优化CAPA-IVM培养条件是必要的,以最大限度地发挥其益处.
- 这些发现为改善人类辅助生殖技术提供了洞察力,以马匹作为模型.
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