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Identification of Small Molecule-binding Proteins in a Native Cellular Environment by Live-cell Photoaffinity Labeling
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使用SLP标签系统在细胞内隔间部位上绘制达沙替尼和其向蛋白之间的结合在活细胞中的图像

Da Kyeong Park1, Sang-Hee Lee1, Hee-Seok Kweon1

  • 1Center for Bio-Imaging & Translational Research and Bioimaging Data Curation Center, Korea Basic Science Institute (KBSI), Cheongju 28119, Republic of Korea.

International journal of molecular sciences
|June 26, 2025
PubMed
概括

研究人员开发了一种简单的方法,可视化活细胞中的药物向相互作用. 这种技术使用自我标记蛋白 (SLP) 标签系统来检测化学药物与标蛋白结合,帮助药物查和精确治疗.

关键词:
在HaloTag中使用HaloTag.在SLP标签系统中,SLP标签系统.达萨提尼布 (dasatinib) 是一种药物目标结合的结合空间局部化的蛋白质表达.亚细胞结构 亚细胞结构

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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