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相关概念视频

RNA Structure01:19

RNA Structure

5.3K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
5.3K
Transfer RNA Synthesis02:36

Transfer RNA Synthesis

12.3K
One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
Each of these chemical modifications is carried by a specific enzyme, post-transcription. All of these enzymes have unique base and site-specificity. Methylation, the most common chemical modification, is carried by at least nine different enzymes, with...
12.3K
Nucleic Acid Structure01:25

Nucleic Acid Structure

7.2K
The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA...
7.2K
Translational Regulation01:29

Translational Regulation

110
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
110
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K
Leaky Scanning02:28

Leaky Scanning

5.2K
During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.2K

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相关实验视频

Updated: Sep 18, 2025

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

31.7K

TARNAS:一种用于抽象和翻译RNA二次结构的软件工具.

Michela Quadrini1, Piero Hierro Canchari1, Piermichele Rosati1

  • 1School of Sciences and Technology, University of Camerino, Via Madonna delle Carceri 7, 62032 Camerino, Italy.

International journal of molecular sciences
|June 26, 2025
PubMed
概括
此摘要是机器生成的。

TARNAS 1.0 是一个新的软件工具,可以在多个格式之间转换RNA二次结构. 它通过促进RNA比较和模式识别,有助于大型数据集的构建和数据库策划.

关键词:
RNA二次结构格式的RNA二次结构格式在RNAML中使用RNAML.这是RNA二次结构的抽象.关于RNA二次结构的统计数据.

更多相关视频

Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.7K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.5K

相关实验视频

Last Updated: Sep 18, 2025

RNA Secondary Structure Prediction Using High-throughput SHAPE
13:42

RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

31.7K
Analyzing and Building Nucleic Acid Structures with 3DNA
16:24

Analyzing and Building Nucleic Acid Structures with 3DNA

Published on: April 26, 2013

20.7K
Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
10:34

Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells

Published on: December 9, 2022

4.5K

科学领域:

  • 生物信息学是一种生物信息学.
  • 计算生物学 计算生物学
  • 分子生物学分子生物学

背景情况:

  • 核糖核酸 (RNA) 折叠成复杂的结构,对它们的生物功能至关重要.
  • RNA二次结构是比较和分类的关键,存在于各种格式.
  • 需要标准化工具来有效处理和分析各种RNA结构.

研究的目的:

  • 介绍TARNAS 1.0,一个用于RNA二次结构格式转换的多功能软件工具.
  • 提供预处理和分析RNA二次结构的能力.
  • 促进大规模RNA数据管理和比较研究.

主要方法:

  • 开发了TARNAS 1.0软件,支持多种RNA二次结构格式 (BPSEQ,CT,点括号,AAS,FASTA,RNAML).
  • 在格式转换期间实施了评论,空白行和标题处理的选项.
  • 集成功能用于计算三级RNA二级结构抽象 (核心,核心加,形状) 和统计描述器.

主要成果:

  • 塔尔纳斯1.0能够在各种RNA二次结构格式之间无转换.
  • 该工具支持通过网络接口进行大型RNA数据集和单分子分析的批处理.
  • TARNAS 1.0 计算结构抽象和统计描述符,以进行增强的RNA比较.

结论:

  • 塔尔纳斯1.0是RNA二次结构数据管理和分析的宝贵工具.
  • 它的格式转换和抽象功能支持大数据集构建和数据库策划.
  • 该软件有助于识别用于将RNA结构与功能关联的结构模式.