丸和卵巢的全长转录组提供了关于Litopenaeus vannamei在性腺发育期间的替代分离的见解
Youyan Wang1, Yang Yu1,2,3, Yue Wang2
1School of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China.
International journal of molecular sciences
|June 26, 2025
概括
太平洋白 (Litopenaeus vannamei) 的性别分化涉及广泛的基因替代拼接 (AS),特别是在卵巢中. 这项研究揭示了新的基因和异型,这些基因和异型对生殖腺发育至关重要.
科学领域:
- 水产养殖的基因组学
- 进行比较的转录学.
- 分子生殖生物学分子生殖生物学
背景情况:
- 太平洋白 (Litopenaeus vannamei) 是一个重要的水产养殖物种,但其性别差异化和淋巴腺发育机制尚未完全理解.
- 以前的研究集中在基因表达上,忽视了性别之间的转录基因结构变异.
- 了解这些过程是改善繁殖的关键,并使无性别繁殖成为可能.
研究的目的:
- 为了对Litopenaeus vannamei丸和卵巢进行全面的全长转录组分析.
- 识别涉及性差异化和性腺发育的新型基因,异型和替代拼接 (AS) 事件.
- 阐明AS在性发育期间的转录后调节中的作用.
主要方法:
- 在Litopenaeus vannamei丸和卵巢的全长转录组测序.
- 生物信息分析用于识别新型基因,异型和替代拼接事件.
- 对男性和女性生殖腺之间的转录结构进行比较分析.
主要成果:
- 鉴定了丸中的830个新基因和卵巢中的690个新基因,以及6000多种新异型.
- 发现了广泛的替代拼接 (AS) 事件,其中一个基因在卵巢中显示了300多种AS异型,而在丸中仅显示了2种.
- 性别决定基因 (Fem-1a,Fem-1c,Sxl) 和生殖细胞发育基因 (MAD2-like,RAD51-like,Su(dx) 呈现出特定于性别的AS模式.
结论:
- 替代拼接在Litopenaeus vannamei的性别分化和生殖腺发育的分子机制中发挥着重要作用.
- 这项研究揭示了新的基因和异型,扩大了繁殖的已知基因组景观.
- 这些发现为AS介导的转录后调节提供了关键的见解,这对于水产养殖的进步至关重要.
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