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从普通话鱼 (Siniperca chuatsi) 中分离的新型伯纳病毒菌株的基因组特征和致病性

Hetong Zhang^1,2, Dandan Zhou³, Junjian Dong^1,2

¹Key Laboratory of Tropical and Subtropical Fisheries Resource Application and Cultivation, Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Institute, Chinese Academy of Fishery Sciences, Guangzhou 510310, China.

|June 26, 2025

概括

一种新的普通鱼比尔纳病毒 (MFBV) 在鱼类中引起急性全身感染,幼更容易受到感染. 这项研究详细介绍了它的基因组,复制和病原性,有助于疾病管理.

科学领域:

水生病毒学水生病毒学.
鱼类疾病研究鱼类疾病研究
分子生物学分子生物学

背景情况:

伯纳病毒会影响经济上重要的鱼类物种.
鱼 (Siniperca chuatsi) 水产养殖在中国具有很高的价值.
需要了解水产养殖中的新型伯纳病毒菌株.

研究的目的:

从普通鱼中分离和描述一种新的比尔纳病毒.
确定新型菌株的基因组和生物特性.
评估不同鱼类阶段的致病性和易感性.

主要方法:

隔离和细胞培养传播 (SCK细胞).
高通量测序和RACE用于基因组确定.
在体外复制,RT-qPCR,组织病理学,以及用于病原性评估的实验挑战.

主要成果:

发现了一种新型菌株MFBV,其基因组分为两部分,与LBBV和LCBV有基因关系.
MFBV迅速复制 (8-10小时周期),在普通鱼,特别是鱼中引起了高死亡率 (41.8-83.6%) 的急性疾病.
在多种组织中检测到病毒RNA;在肠道,脏和脏中观察到病变. 对于甲敏感的MFBV.

结论:

在普通鱼中,MFBV引发了急性系统性感染.
基因组和生物数据提供了对比尔纳病毒多样性的见解.
这些发现支持改善疾病管理策略和病毒分类学.

关键词:

一个比尔纳病毒.消毒剂消毒剂消毒剂消毒剂鱼是一个普通话鱼.病原性病原性病原性

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