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使用高分辨率质谱法 (UHPLC-HRMS) 对特征性牛奶进行非向性脂组学分析

Tingting Wei¹, Tianxiao Zhou¹, Shenping Zhang¹

¹Key Laboratory of Milk and Dairy Products Detection and Monitoring Technology, State Administration for Market Regulation, Shanghai Institute of Quality Inspection and Technical Research, Shanghai 200233, China.

Foods (Basel, Switzerland)

|June 26, 2025

概括

这项研究揭示了8种动物物种的独特牛奶脂质概况,使用非向的脂质组学. 这些发现突出了针对定制功能乳制品开发和地理标志可追溯性的独特脂质组成.

科学领域:

* 食品科学与技术 * 食品科学与技术
* 营养生物化学 * 营养生物化学
* 分析化学分析化学

背景情况:

* 牛奶脂对于乳制品的营养价值,功能和加工至关重要.
*了解不同的牛奶脂质概况对于开发专门的乳制品应用是必不可少的.
* 特定物种的脂质组成可以提供独特的健康和加工益处.

研究的目的:

* 系统地描述和比较来自八种动物的十种牛奶类型的脂质特征.
* 为了识别特定物种的脂质特征和潜在的生物标志物.
*为开发定制,功能和区域品牌乳制品提供分子洞察力.

主要方法:

*非向性脂管学方法采用超高性能液态染色学与超高性能液态染色学-高分辨率质谱学 (UHPLC-HRMS) 相结合.
* 识别和量化640种脂类物种,包括甘油三 (TGs),脂 (PLs),脂 (SPs),陶 (Cer) 和 Ester (WE).
* 统计分析包括主要成分分析 (PCA),层次聚类和火山图片,以确定显著差异和确定生物标志物.

主要成果:

* 在十个牛奶样本中观察到脂质类型和丰度的显著差异.
*驼牛奶具有丰富的脂和脂的高脂多样性;马牛奶富含多不和脂肪酸 (PUFA).
*驴牛奶富含胆固醇和PUFA;山羊牛奶中链脂肪酸 (MCFA) 含量较高;水牛牛奶含TG和酸 (WE) 含量较高;雅克牛奶含有较高的胺 (Cer) 和和脂肪酸.
* 泽西和霍尔斯坦牛奶表现出类似的,稳定的脂质特征.
* 确认了特定物种的脂质组特征,并确定了LPC (O-16:0) 等潜在生物标志物,用于地理标志 (GI) 的追溯性.

结论:

* 建立了跨多种牛奶来源的综合性脂质基因格局.
* 独特的脂质配置文件为开发基于动物物种的专用乳制品提供了分子洞察力.
*可利用脂质组特征来实现乳制品中的地理标志 (GI) 追溯性.

关键词:

在UHPLC-HRMS中使用.具有特征性的牛奶牛奶.功能性脂质是一种功能性脂质.脂质组成脂质组成脂质组的类型是什么脂质是一种脂质.三甲基甘油三甲基甘油三甲基甘油

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