烤提高了来自高地大麦的多的生物可访问性和生物活性,对氧化损伤的HepG2细胞具有保护作用
Nuo Chen1, Shuyu Pang1, Xingru Zao2
1College of Food Science and Technology, Yunnan Agricultural University, Kunming 650201, China.
Foods (Basel, Switzerland)
|June 26, 2025
概括
烤高地大麦 (HB) 显著提高了多的生物可访问性和抗氧化活性. 这增强了肝细胞对氧化应激的保护,为新的功能性食物铺平了道路.
科学领域:
- 食品科学 食品科学 食品科学
- 营养生物化学 营养生物化学
- 细胞生物学 细胞生物学
背景情况:
- 多是具有抗氧化特性的生物活性化合物.
- 高地大麦 (HB) 是有益多的来源.
- 烤是一种常见的食品加工技术,可以改变植物化学特征.
研究的目的:
- 调查烤对高地大麦的多释放,生物可访问性和生物活性的影响.
- 评估烤HB多提取物对HepG2细胞中氧化应激的抗氧化和保护作用.
主要方法:
- 实验室消化和结肠发酵模型被用于评估多的生物可访问性.
- 测量了酶抑制活性 (α-氨基酶,α-葡萄糖酶,脂酶).
- 在HepG2细胞中建立了过氧化 (H2O2) 诱导的氧化应激模型.
- 烤HB多提取物对细胞氧化损伤的保护作用通过测量活性氧物种 (ROS),甲酸 (MDA) 和抗氧化酶活性 (SOD,GSH,CAT,GPx,T-AOC) 来评估.
主要成果:
- 烤在胃肠道消化和结肠发酵过程中显著增加了多的生物可访问性.
- 烤的HB多醇表现出对α-氨基酶,α-葡萄糖酶和脂酶的增强抑制活性.
- 从烤HB中提取的聚烯提取物降低了HepG2细胞中的ROS和MDA水平.
- 烤HB多提取物增加了关键抗氧化酶 (SOD,GSH,CAT,GPx) 和总抗氧化能力 (T-AOC) 的活性.
结论:
- 烤可以提高高地大麦中的多的生物可访问性和生物活性.
- 烤HB多提供显著的抗氧化和细胞保护作用,对抗氧化应激.
- 这些发现支持开发功能性食品,其中包括烤高地大麦的健康益处.
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