相关实验视频

Updated: Apr 13, 2026

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Generation of Genomic Deletions in Mammalian Cell Lines via CRISPR/Cas9

Published on: January 3, 2015

使用CRISPR/Cas9技术生成Plexin-B1条件淘汰赛小鼠

Haofei Ni^1,2, Kevin Kelley³, Ning Xie¹

¹Division of Spine, Department of Orthopedics, Tongji Hospital, Tongji University School of Medicine, Shanghai, China.

Genesis (New York, N.Y. : 2000)

|June 26, 2025

概括

研究人员使用CRISPR/Cas9.1生成了一个有条件的Plexin-B1 (Plxnb1) 鼠标模型. 这种新的Plxnb1-floxed等位基因允许对Plexin-B1在发育和生理学中的功能进行细胞类型特定研究.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

素是关键的轴突引导受体,参与细胞骨和膜动力学.
它们的广泛表达需要细胞类型特定的功能分析工具.

研究的目的:

在小鼠中产生Plexin-B1 (Plxnb1) 的条件流动性等位基因.
为了使Plxnb1.1.的未来细胞类型特定的功能研究成为可能.

主要方法:

使用CRISPR/Cas9技术,插入了Plxnb1基因关键外显子旁边的loxP位点.
向试剂通过电穿孔输入单细胞小鼠胚胎.
使用PCR和桑格测序进行查和验证.

主要成果:

在小鼠中成功生成了一个有条件的Plxnb1-floxed等位基因,成功率约为1.6%.
Alt-R 修改后的 ssODNs 提高了一个 loxP 站点的定位效率.
这种Plxnb1-floxed等位基因是通过生殖线传播的,并使用Cre recombinase有效地切除.

结论:

使用CRISPR/Cas9.9创建了一个有条件的Plxnb1-floxed小鼠模型.
该模型为研究Plexin-B1在各种生物环境中的特定作用提供了有价值的工具.

更多相关视频

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System

Generation of a RIP1 Knockout U937 Cell Line Using the CRISPR-Cas9 System

Published on: April 11, 2025

相关概念视频

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.