自主激活和染色体同位素的LsERF166积极调节了生菜中的costunolide生物合成
Yuting Xiang1, Shuxin Zheng1, Bang Chen1
1Key Laboratory of Bio-resource and Eco-environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu, 610064, China.
The New phytologist
|June 26, 2025
概括
研究人员确定了一个关键的调节器,乙烯反应因子166 (LsERF166),它控制了生菜中的costunolide生物合成. 这种转录因子激活基因表达,形成一个积极的反循环,增强这种重要的天然产品的生产.
科学领域:
- 植物分子生物学 植物分子生物学
- 生物化学 生物化学
- 基因组学就是基因组学.
背景情况:
- 科斯图诺利德是一种重要的天然产品,也是Asteraceae植物中sessquiterpene乳的前体.
- 控制costunolide生物合成的监管网络尚未得到充分理解.
- 乳 (Lactuca sativa) 是一个适合的模型,研究由于其丰富的sessquiterpene乳生物合成.
研究的目的:
- 为了研究菜中costunolide生物合成的转录调节.
- 确定涉及控制costunolide生产的关键转录因子.
- 阐明成本化物生物合成背后的调节机制.
主要方法:
- 为生菜构建一个多组织转录组数据集.
- 对costunolide生物合成基因的联合表达分析.
- 染色体局部化研究以确定物理相邻的调节元素.
- 乙烯反应因子 (ERF) 转录因子的识别和表征.
主要成果:
- 科斯图诺利德生物合成基因在不同的生菜组织中共同表达.
- 鉴定出LsERF166,一种可诱导乙烯的ERF IX转录因子,在物理上与costunolide synthase (COS) 基因相邻.
- LsERF166直接激活了costunolide生物合成基因的表达,积极调节生物合成.
- LsERF166表现出自我激活,形成一个积极的反循环.
- 在多种Asteraceae物种中观察到ERF166和COS基因的局部化.
结论:
- LsERF166是一种自我激活的转录因子,在调节costunolide生物合成中起着重要作用.
- LsERF166和COS基因的物理局部化表明,Asteraceae中存在一种保存的调节机制.
- 这项研究为植物二次新陈代谢的转录控制提供了新的见解,特别是costunolide生产.
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