呼吸和血液代谢学:使用SESI-HRMS/MS和UHPLC-ESI-HRMS/MS进行比较研究
Zhifeng Tang1,2, Jianming Yang3, Xin Xu4
1College of Environment and Climate, Institute of Mass Spectrometry and Atmospheric Environment, Guangdong Provincial Key Laboratory of Speed Capability Research, Jinan University, Guangzhou 510632, China.
Environment & health (Washington, D.C.)
|June 26, 2025
概括
呼吸代谢学,使用二次电喷离子化高分辨率联质谱 (SESI-HRMS/MS),提供独特的代谢洞察力补充血液分析. 这种方法增强了生命科学中的生物标志物发现.
科学领域:
- 代谢学 代谢学 代谢学
- 分析化学 分析化学
- 生物化学 生化学
背景情况:
- 呼吸代谢学非侵入性地检测出口气中的挥发性有机化合物 (VOC).
- 二次电喷射电离高分辨率联质谱 (SESI-HRMS/MS) 是一项领先的实时呼吸分析技术.
- 了解呼吸和血液代谢物之间的分子关系至关重要,但有限.
研究的目的:
- 使用先进的质谱技术,比较呼吸和血液中的代谢物.
- 研究共享和独特代谢物的相关性和特征.
- 评估呼吸和血液代谢的互补性,以发现生物标志物.
主要方法:
- 来自11名健康志愿者的呼吸和血液样本的非目标分析.
- 使用SESI-HRMS/MS进行呼吸和UHPLC-ESI-HRMS/MS进行血液分析.
- 采用双重质谱法进行代谢物注释,并确定了独特和共享的代谢物.
主要成果:
- 确定了26种独特的呼吸代谢物和73种独特的血液代谢物.
- 在呼吸和血液中检测到7种代谢物,相关性较低 (r < 0.4).
- 呼吸代谢物显示出更高的挥发性;性别差异在呼吸中不如血液中明显.
结论:
- 呼吸代谢产物为血液代谢产物提供补充信息.
- 结合呼吸和血液代谢学,可以增强生物标志物的发现.
- 这项研究支持呼吸代谢学在生命科学中的应用.
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