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相关概念视频

Enlargement of the Plasma Membrane01:22

Enlargement of the Plasma Membrane

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Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
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Updated: May 6, 2026

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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可扩展的膜启用了一液相寡核酸合成.

Ronan Kelly1,2, Catalina Parga1,2, Steven Ferguson1,2,3

  • 1Department of Chemical and Bioprocess Engineering, University College Dublin, D04 V1W8 Dublin 4, Ireland.

Organic process research & development
|June 26, 2025
PubMed
概括

一种新的单液相寡核酸合成 (OP-LPOS) 方法使用耐有机溶剂的陶膜进行高效的生产. 这种方法提供了高产量和纯度,为治疗性寡核酸的传统固相合成提供了可扩展的替代方案.

关键词:
液相寡核酸合成液相寡核酸合成膜膜膜膜是一种纳米过的纳米过方法聚核酸的治疗药物.有机溶剂有机溶剂超过是一种超过.

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科学领域:

  • 化学工程是化学工程的重要组成部分.
  • 生物技术是生物技术.
  • 材料科学 材料科学 材料科学

背景情况:

  • 固相寡核酸合成 (SPOS) 是目前治疗性寡核酸生产的标准.
  • SPOS方法通常涉及漫长的过程,大量的试剂浪费,以及扩大规模的挑战.
  • 需要更高效和可持续的方法来制造寡核酸.

研究的目的:

  • 引入一种新的一液相寡核酸合成 (OP-LPOS) 途径.
  • 为了证明在OP-LPOS中耐有机溶剂 (OSR) 陶膜的有效性.
  • 为治疗性寡核酸生产提供可扩展和高效的SPOS替代方案.

主要方法:

  • 使用有机溶剂纳米过 (OSN) 和超过 (OSU) 陶膜.
  • 开发了一种集结合,硫化和脱细化步骤的单工艺.
  • 采用了一个可逆的绑定策略,将生长的寡核酸结合到分支的PEG支.

主要成果:

  • 对于6mer和18mer寡核酸,取得了较高的阶段性过产量 (97-100%).
  • 使用最小的酸化物 (1.5 equiv) 获得高纯度原油 (∼72%对于18mer).
  • 在5个具有高膜通透度 (13 Lm-2 h-1 bar-1) 的方体中证明了高效的净化.

结论:

  • 带有OSR陶膜的OP-LPOS是用于大规模制造的SPOS的一个有希望的替代品.
  • 这种方法提高了中间产品的纯度和产量,同时减少了单元操作和循环时间.
  • 该过程利用可扩展的膜系统和商用试剂,提高了工艺质量强度 (PMI).