Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Determination of Crystal Structures01:29

Determination of Crystal Structures

139
In the late 1800s, the revelation that light extended beyond visible wavelengths led to the discovery of X-rays by Wilhelm Roentgen. Recognized as high-energy electromagnetic radiation with short wavelengths, X-rays prompted exploration into their interaction with crystals. Max von Laue proposed in 1912 that the periodic arrangement of atoms, ions, or molecules in crystals would cause them to diffract X-rays, a hypothesis confirmed through experiments with copper sulfate and zinc sulfide...
139

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mesoglea biogenesis reveals a cryptic aboral valve for pressure regulation in cnidarian morphogenesis.

Science advances·2026
Same author

Radiation-induced peroxide rupture and its temperature-dependent repair probed by homogeneous X-ray irradiation.

Acta crystallographica. Section D, Structural biology·2026
Same author

Radiation damage in sub-Ångström resolution macromolecular crystallography: a low-dose study.

Acta crystallographica. Section D, Structural biology·2026
Same author

CryoRhodopsins: A comprehensive characterization of a group of microbial rhodopsins from cold environments.

Science advances·2025
Same author

Author Correction: DNA-guided transcription factor interactions extend human gene regulatory code.

Nature·2025
Same author

Proteorhodopsin insights into the molecular mechanism of vectorial proton transport.

Science advances·2025

相关实验视频

Updated: May 6, 2026

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Published on: March 19, 2019

5.9K

增强了基于双网格扫描的多晶数据收集功能.

Igor Melnikov1, Olof Svensson1, Gleb Bourenkov2

  • 1European Synchrotron Radiation Facility, BP 220, 38043 Grenoble, France.

Acta crystallographica. Section D, Structural biology
|June 26, 2025
PubMed
概括

升级后的DoubleMesh&Collect方法使用两个X射线网状扫描来精确地定位3D晶体和评估宏分子晶体学中的质量,从而提高数据收集效率.

关键词:
在这里,Dozor Dozor.在 Dozor-m2 的位置上.这就是Resheteau.雷射网格格格子扫描扫描的X射线.数据收集策略数据收集策略.宏分子晶体学 宏分子晶体学样本本地化的样本定位

更多相关视频

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
07:11

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Published on: March 22, 2019

7.0K
Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

3.4K

相关实验视频

Last Updated: May 6, 2026

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
06:42

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Published on: March 19, 2019

5.9K
Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules
07:11

Fully Autonomous Characterization and Data Collection from Crystals of Biological Macromolecules

Published on: March 22, 2019

7.0K
Fixed Target Serial Data Collection at Diamond Light Source
06:19

Fixed Target Serial Data Collection at Diamond Light Source

Published on: February 26, 2021

3.4K

科学领域:

  • 晶体学 晶体学是指结晶学.
  • 结构生物学 结构生物学
  • 在X射线中,X射线的衍射.

背景情况:

  • 二维X射线网状扫描是定位样本位置在宏分子晶体学中的标准.
  • 该 Mesh&Collect 方法使用单网状扫描来收集来自多个晶体的衍射数据.
  • 以前的方法在准确确定晶体位置和尺寸方面存在局限性.

研究的目的:

  • 为改善晶体表征提供一个增强的DoubleMesh&Collect方法
  • 为了能够更准确地确定3D晶体中心和尺寸.
  • 优化宏分子晶体学中衍射数据收集策略.

主要方法:

  • 使用两个X射线网状扫描,通常在直角方向.
  • 使用Dozor,Dozor-m2和Resheteau软件进行自动化分析.
  • 分析来自双网状扫描的衍射图像,以确定晶体参数.

主要成果:

  • 证明了对3D晶体中心位置的准确确定.
  • 提供了对晶体尺寸和衍射品质的改进估计.
  • 成功地将该方法应用于各种情况下,包括室温研究.

结论:

  • 双网格和收集方法比单网格扫描提供了更高的准确性.
  • 这种升级的方法提高了宏分子结晶学实验的效率和可靠性.
  • 进一步的讨论涉及扫描区域内大型晶体的绘图.