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针对三阴性乳腺癌治疗的aptamer导向siRNA传递系统

Dilpreet Singh¹, Satvir Singh², Nitin Tandon²

¹School of Pharmaceutical Sciences, CT University, Punjab, India.

Nucleosides, nucleotides & nucleic acids

|June 26, 2025

概括

阿普塔默-siRNA结合物为三阴性乳腺癌 (TNBC) 提供了一种新的基因沉默疗法. 这些向疗法显示出显著的瘤减少和比传统治疗更好的疗效.

科学领域:

生物化学生物化学
分子生物学分子生物学
在瘤学瘤学.

背景情况:

三阴性乳腺癌 (TNBC) 缺乏向疗法,需要新的治疗策略.
对TNBC的化疗和放射治疗的疗效有限,副作用严重.
阿普塔默-siRNA结合物代表了TNBC的一个有前途的基因沉默方法.

研究的目的:

审查TNBC的阿普坦-siRNA治疗方法的机制,进展,挑战和未来方向.
突出阿普坦-siRNA结合物的潜力,作为一个有针对性和有效的基因沉默策略.
分析基于aptamer-siRNA的疗法的临床翻译前景.

主要方法:

通过SELEX生成的aptamers特别与TNBC生物标志物 (例如EGFR,EpCAM) 结合.
阿普塔默-siRNA结合物促进受体介导的细胞吸收和向的siRNA递送.
在异种移植模型中进行的临床前研究评估了治疗疗效和药理动力学.

主要成果:

阿普塔默-siRNA结合物证明了5-10倍的增强细胞吸收和80-95%的基因沉默效率.
循环中的siRNA稳定性从<2小时增加到6-9小时.
在异种移植模型中观察到60-85%的瘤体积减少,表现优于对照组.

结论:

阿普塔默-siRNA结合物为TNBC提供了有针对性,有效和潜在的毒性较小的治疗策略.
克服核酶降解和免疫反应等挑战对于临床翻译至关重要.
体设计和输送系统的进步为基于RNAi的个性化TNBC疗法铺平了道路.

关键词:

亚普塔默-siRNA结合物是一种结合物.通过RNA干扰 (RNAi) 疗法进行治疗.基于纳米载体的药物输送.精确瘤学精确瘤学有针对性的基因沉默.三重阴性乳腺癌 (TNBC) 是一种

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