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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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相关实验视频

Updated: Sep 18, 2025

Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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MEBOCOST使用单细胞RNA序列绘制了代谢物介导的细胞间通信图.

Rongbin Zheng1,2, Yang Zhang3,4, Tadataka Tsuji3,4

  • 1Department of Cardiology, Boston Children's Hospital, Boston, MA, 02115, United States.

Nucleic acids research
|June 26, 2025
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概括

MEBOCOST是一种新的算法,用于检测使用单细胞RNA测序和代谢建模的代谢物介导细胞-细胞通信 (mCCC). 它确定了肥胖和脂肪细胞分化中的关键mCCC相互作用,进步了我们对代谢调节的理解.

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相关实验视频

Last Updated: Sep 18, 2025

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科学领域:

  • 代谢学 代谢学 代谢学
  • 系统生物学 系统生物学
  • 一个单细胞分析.

背景情况:

  • 细胞-细胞通信 (CCC) 对于组织功能至关重要.
  • 现有的方法往往错过了代谢物介导的CCC (mCCC).
  • 为mCCC调整以蛋白质为中心的方法是很困难的.

研究的目的:

  • 开发一种用于检测mCCC的计算工具.
  • 在人类肥胖和小鼠棕色脂肪组织中分析mCCC.
  • 确定调节脂肪细胞分化的新型mCCC通路.

主要方法:

  • 开发了集scRNA-seq和代谢流量平衡分析的MEBOCOST算法.
  • 使用模拟,空间,CRISPR屏幕和临床数据验证了MEBOCOST.
  • 将MEBOCOST应用于人类白色脂肪组织和小鼠棕色脂肪组织数据集.

主要成果:

  • 梅博科斯特证明了对显著的mCCC事件的稳健检测.
  • 确定巨细胞是肥胖患者mCCC重编程的主要来源.
  • 发现了一种新的谷氨胺介导的内皮细胞与脂肪细胞之间的通信,调节脂肪细胞的分化.

结论:

  • 梅博科斯特是研究mCCC在各种生物环境中的强大工具.
  • 提供了关于肥胖的代谢失调的见解.
  • 有助于发现和实验验证新的mCCC机制.