酸盐缺乏通过酸盐饥饿反应减少结节的形成 - - 类似于Phaseolus vulgaris中的蛋白质
Jawahar Singh1, Ana Belén Mendoza-Soto1, Manish Tiwari2
1Laboratorio de Genómica Funcional de Leguminosas, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Tlalnepantla 54090, México.
Plant & cell physiology
|June 26, 2025
概括
酸盐缺乏通过激活自调节结节 (AON) 途径,影响豆类结节的形成. 这项研究确定了PvPHR-L7作为一个关键的转录因子,在不同的酸盐条件下调节Phaseolus vulgaris中结节的发展.
科学领域:
- 植物分子生物学 植物分子生物学
- 豆类的共生关系是豆类的共生关系.
- 营养信号传递 营养信号传递
背景情况:
- 酸盐缺乏会对豆类结节的形成,固和作物产量产生负面影响.
- 酸盐缺乏激活了结节自律 (AON) 途径,影响结节的发展.
- PHR1转录因子与P1BS的cis调节元件结合,这表明它在酸盐调节的基因表达中起作用.
研究的目的:
- 为了研究PvPHR-Like 7 (PvPHR-L7) 在调节Phaseolus vulgaris中的结节形成中的作用.
- 阐明宿主植物酸盐水平通过PHR-Like蛋白质控制结节发育的机制.
主要方法:
- 通过RNA干扰 (RNAi) 和过度表达来调节PvPHR-L7的表达.
- 用RT-qPCR在不同的酸盐条件下分析基因表达.
- 在Nicotiana benthamiana和转基因P. vulgaris根的交易活化试验.
主要成果:
- 调节PvPHR-L7表达会影响共生基因PvNIN和PvTML的表达.
- PvPHR-L7,PvNIN和PvTML的表达水平与植物宿主酸盐 (Pi) 的水平相关.
- 在没有根茎菌的情况下,PvPHR-L7可对PvNIN和PvTML进行上调,但在共生过程中可对PvNIN进行下调.
结论:
- PvPHR-L7 是一个关键的转录因子,参与调节Phaseolus vulgaris中结节的形成.
- PvPHR-L7在结节共生中起着双重作用,根据根茎生物的存在和酸盐的可用性差异性调节关键基因.
- 这项研究提供了关于在营养压力下控制根结节共生的分子机制的见解.
相关概念视频
Key Elements for Plant Nutrition
21.1K
Like all living organisms, plants require organic and inorganic nutrients to survive, reproduce, grow and maintain homeostasis. To identify nutrients that are essential for plant functioning, researchers have leveraged a technique called hydroponics. In hydroponic culture systems, plants are grown—without soil—in water-based solutions containing nutrients. At least 17 nutrients have been identified as essential elements required by plants. Plants acquire these elements from the...
21.1K
Protein Kinases and Phosphatases
13.4K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
13.4K
Phosphorylation
51.1K
The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
51.1K
Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
Roles of Electrolytes: Calcium and Phosphate
479
Calcium and phosphate are essential electrolytes in the human body, with calcium being the most abundant mineral. Around 99% of the body's calcium is stored in the skeleton and teeth, forming a crystal lattice of mineral salts in combination with phosphates. Calcium plays crucial roles in various bodily functions such as blood clotting, neurotransmitter release, muscle tone maintenance, and nervous and muscle tissue excitability.
The calcium concentration in blood plasma is primarily...
The calcium concentration in blood plasma is primarily...
479
The Roles of Bacteria and Fungi in Plant Nutrition
40.6K
Plants have the impressive ability to create their own food through photosynthesis. However, plants often require assistance from organisms in the soil to acquire the nutrients they need to function correctly. Both bacteria and fungi have evolved symbiotic relationships with plants that help the species to thrive in a wide variety of environments.
40.6K


