相关实验视频

Updated: Aug 14, 2026

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

来自CAF的LRRC15调节了巨细胞的两极分化,并限制了PD-1免疫治疗在质母细胞瘤中的疗效

Feifei Luo^1,2, Yan Mei³, Yanwen Li¹

¹Department of Neurosurgery, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, China.

|June 26, 2025

概括

在癌症相关纤维细胞 (CAF) 中准LRRC15可以通过重编程瘤微环境 (TIME) 和增强抗瘤疗效来克服质母细胞瘤 (GBM) 对PD-1免疫疗法的抵抗力.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物信息学是一种生物信息学.

背景情况:

质母细胞瘤 (GBM) 由于其免疫抑制瘤微环境 (TIME),对PD-1/PD-L1免疫疗法的反应有限.
将TIME重新编程到促炎状态,为GBM提供了一个潜在的治疗策略.

研究的目的:

研究癌症相关纤维细胞 (CAF) 和LRRC15表达在GBM免疫疗法耐药性中的作用.
确定新的治疗点,以克服GBM中抗PD-1疗法的耐药性.

主要方法:

单细胞RNA测序 (scRNA-seq) 和空间转录组学 (stRNA-seq) 数据的生物信息学分析.
在体外和体外对LRRC15分子机制的功能验证.
在CAF和组合治疗研究中对Lrrc15的基因操纵.

主要成果:

在不响应抗PD-1疗法的患者中,确定了具有高LRRC15表达的特定CAF子集群.
在CAF中的LRRC15通过FAK/SRC/NF-κB通路促进M2巨分极和IL8表达,推动瘤生长.
在CAF中删除Lrrc15和与抗PD-1增强抗瘤功效的联合治疗.

结论:

准LRRC15表达CAF是一种有前途的策略,可以提高在GBM中抗PD-1疗法的疗效.
这种方法可以通过调节时间来克服GBM的免疫疗法耐药性.

关键词:

对于LRRC15来说,这是一个很大的问题.与癌症相关的纤维细胞细胞.质母细胞瘤 (glioblastoma) 是一种免疫疗法耐药性免疫疗法耐药性与瘤相关的巨细胞.

更多相关视频

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Coculture Assays to Study Macrophage and Microglia Stimulation of Glioblastoma Invasion

Published on: October 20, 2016

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.