病原病毒的自然自我减弱通过删除长期植物病毒共存的沉默抑制器编码序列来减弱病原病毒的自然自我减弱
Li Qin1, Xiaoqing Wang1, Zhaoji Dai1
1Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests (Ministry of Education), School of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
PLoS pathogens
|June 26, 2025
概括
在Arepavirus属中的Potyviridae病毒可以失去领导蛋白酶基因,将症状从严重的亡转移到轻微的化. 这种自我减弱允许病毒和多年宿主植物之间长期共存.
科学领域:
- 植物病毒学 植物病毒学
- 分子生物学分子生物学
- 在RNA病毒的演化过程中.
背景情况:
- 波提病毒科 (Potyviridae) 是最大的植物RNA病毒家族,利用聚蛋白加工.
- 领导蛋白酶 (P1和HCPro) 对病毒的功能至关重要,包括RNA沉默抑制和传播.
- 阿雷帕病毒属病毒编码两个并联的HCPro领导蛋白酶 (HCPro1-HCPro2).
研究的目的:
- 为了研究Arepavirus中领导蛋白酶编码序列的自然损失.
- 了解这种基因组删除对病毒与宿主相互作用和症状发展的功能后果.
- 探索类病毒的自我减弱的进化含义.
主要方法:
- 自然发生的删除突变体的基因组分析.
- 反向遗传学证实了领导蛋白酶的功能.
- 实地调查以评估Areca棕树的病毒种群和症状严重程度.
主要成果:
- 发现阿雷帕病毒分离物在感染期间自然失去HCPro1-HCPro2编码序列.
- 领导蛋白酶的丧失与从严重亡转向轻微化或无症状感染的转变相关.
- 侧边重复序列之间的重组可能驱动基因组删除.
- 删除突变体表现出弱化的RNA沉默抑制和其他关键功能.
结论:
- 领导蛋白质酶的损失代表了病毒体自我减弱的新机制.
- 这一过程可以通过减轻损害,使多年宿主和病毒之间长期共存.
- 全长和删除突变病毒在受感染的树木中共存,在感染和传播中发挥着不同的作用.
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