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相关概念视频

Master Transcription Regulators02:23

Master Transcription Regulators

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Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
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Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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General Transcription Factors01:30

General Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Combinatorial Gene Control02:33

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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相关实验视频

Updated: Sep 18, 2025

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
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基因调节程序指定了在胸细胞发育过程中与年龄相关的差异.

Divya Ganapathi Sankaran1, Hongya Zhu1, Viviana I Maymi2

  • 1Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.

Cell reports
|June 26, 2025
PubMed
概括
此摘要是机器生成的。

这项研究揭示了T细胞发育如何随着年龄的增长而变化,通过创建基因图谱. 一个关键的调节器,Zbtb20,影响这些T细胞程序的年龄相关差异.

关键词:
CD8 (((+) T细胞是T细胞中的一个.CP: 免疫学 免疫学在HSCs中,HSCs是最重要的.在RNA-seqqq.在T细胞发育过程中,成年人成年人成年人成年人成年人这是一个双负的负面.双重阳性 双重阳性 双重阳性免疫系统是免疫系统的.新生儿们的新生儿一个单一的正面结果.

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科学领域:

  • 免疫学 免疫学 免疫学
  • 发展生物学 发展生物学
  • 基因组学就是基因组学.

背景情况:

  • 对免疫功能来说,T细胞的发育至关重要.
  • 与T细胞发育的年龄相关的变化尚不清楚.

研究的目的:

  • 为了研究T细胞发育中的年龄相关差异.
  • 确定基因调节程序和参与这些变化的调节者.

主要方法:

  • 在新生儿和成年小鼠中构建了T细胞发育的转录和染色质可访问性地图.
  • 利用基于CRISPR的扰动和单细胞RNA测序.
  • 分析了不同的基因调节程序和染色质可访问性.

主要成果:

  • 确定了一种影响效应因子响应和细胞周期的年龄分离基因模块.
  • 在新生儿胸细胞发育中观察到更容易获得的染色体,这表明基因表达平衡.
  • 发现Zbtb20作为一种保存的转录调节剂,有助于年龄相关的差异.

结论:

  • 定义了基因调节程序,为特定年龄的T细胞发育提供了基础.
  • 强调了染色质可访问性在早期发育中的作用.
  • 确定Zbtb20作为与年龄相关的T细胞成熟的关键因素.