冷大气等离子衍生的ROS解毒脱氧尼瓦诺诱导的神经毒性
Ruonan Ma1, Junxia Feng2, Hangbo Xu1
1Zhengzhou Research Base, National Key Laboratory of Cotton Bio-breeding and Integrated Utilization, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China; Henan Key Laboratory of Ion-beam Green Agriculture Bioengineering, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, China; Sanya Institute, Zhengzhou University, Zhengzhou 450001, China.
冷大气等离子体 (CAP) 有效降解脱氧尼瓦伦醇 (DON),一种导致脏损伤的谷物污染物. CAP治疗通过保护细胞和减少 in vivo损伤标志物来减轻DON诱导的毒性.
科学领域:
- 食品科学与技术 食品科学与技术
- 毒理学 毒理学 毒理学
- 生物化学 生物化学
背景情况:
- 脱氧尼瓦伦醇 (DON) 是一种普遍存在的谷物菌毒素,在人类和动物中引起严重的损伤.
- 目前对DON的排毒策略有限,其精确的毒性机制需要进一步阐明.
- 冷大气等离子体 (CAP) 呈现了一个有前途的,富含反应性氧物种的技术,用于毒素去污染.
研究的目的:
- 研究CAP对脱氧尼瓦伦醇诱导的毒性减轻效应.
- 通过CAP探索DON降解途径,并评估其副产品的毒性.
- 阐明DON毒性和CAP的保护作用背后的细胞机制.
主要方法:
- 通过CAP分析了DON的降解,确定了反应性物种 (1O2, ∙OH, ∙O2-, H2O2) 和产品.
- 在实验室中,使用人类脏邻近管状上皮细胞 (HKC-8) 评估了毒性,而在体内研究中使用了小鼠急性损伤模型.
- 机理学研究侧重于氧化应激和亡途径,包括Nrf2/HO-1/NQO-1信号和JNK介导的Nrf2降解.
主要成果:
- 在120秒内,CAP处理降解了超过98%的DON,通过改变有毒的功能群产生了5种不同的降解产品.
- 在体外和体内,DON诱导了显著的毒性,由细胞活力降低,血清肌素升高和血尿素 (BUN) 水平升高所证明.
- 用CAP治疗的DON (Pla-DON) 逆转了线粒体损伤,并通过抑制JNK介导的Nrf2降解,显著改善细胞活力和减少损伤标志物来维持还原平衡.
结论:
- CAP有效降解了DON并减轻了与之相关的毒性.
- 通过对Nrf2信号通路的调节,CAP通过保护线粒体功能和细胞内氧化还原平衡来保护细胞.
- 这项研究强调了农业政策作为一种有效的策略,用于消毒谷物和预防DON引起的脏损伤.
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