相关实验视频

Updated: Sep 18, 2025

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Isolation, Characterization and MicroRNA-based Genetic Modification of Human Dental Follicle Stem Cells

Published on: November 16, 2018

长非编码RNA MIR4435-2HG 参与人类牙髓干细胞的骨质分化和炎症

Feijun Wu¹, Zhisheng Jiang²

¹Department of Paediatric Stomatology, Changsha Stomatological Hospital, Changsha, Hunan, China.

International dental journal

|June 26, 2025

概括

长非编码RNAMIR4435-2宿主基因 (MIR4435-2HG) 通过调节miR-296-5p抑制牙纸细胞的骨质分化,并促进炎症,为皮炎提供治疗见解.

科学领域:

口腔生物学口腔生物学
分子生物学分子生物学
干细胞研究的研究.

背景情况:

胸腔炎是一种普遍的口腔疾病,需要对其分子机制有更深入的了解.
识别 Pulpitis 和牙组织再生的新疗法目标至关重要.

研究的目的:

研究长非编码RNAMIR4435-2宿主基因 (MIR4435-2HG) 在人类牙髓干细胞 (hDPSC) 的骨质分化中的作用.
阐明MIR4435-2HG在hDPSCs的炎症反应中的作用.
探索MIR4435-2HG作为一个潜在的治疗的目标.

主要方法:

使用实时定量PCR对纸组织中MIR4435-2HG表达的量化.
使用hDPSC进行体外研究,以评估MIR4435-2HG对骨质分化和脂多糖 (LPS) 诱导的炎症的影响.
细胞活力,亡测定和双路西法酶记者基因测定以确认与miR-296-5p的向关系.

主要成果:

在炎症的肉皮组织中,MIR4435-2HG的表达被上调.
在hDPSC中,MIR4435-2HG抑制了骨质生成分化标志物和性酸酶活性.
在经过miR-296-5p调解的LPS治疗的hDPSC中,MIR4435-2HG的抑制降低了炎症媒介,促进了增殖,并抑制了亡.

结论:

通过调节miR-296-5p,MIR4435-2HG抑制骨质分化并促进hDPSCs中的炎症.
这些发现凸显了MIR4435-2HG在脉病原发生中的重要作用.
MIR4435-2HG是牙组织修复和脉炎治疗的潜在治疗标.

关键词:

炎症炎症是一种炎症.在MIR4435-2HGG中使用.骨质变异的分化骨质变异的分化讲堂的教堂 (pulpitis) 是一个教堂.在 miR-296-5p 中.

更多相关视频

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods

Published on: November 24, 2012

Accessing the Cytotoxicity and Cell Response to Biomaterials

Accessing the Cytotoxicity and Cell Response to Biomaterials

Published on: July 8, 2021

相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...