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Updated: Sep 18, 2025

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TAS-seq通过信号辅助的腺去氨化来实现亚细胞单链腺分析

Lixia Wang1, Yangfan Zhou2, Zhenxing Yu2

  • 1Fudan University, 220 Handan Road, Shanghai 200433, China; Westlake Laboratory, School of Life Sciences, Westlake University, 600 Dunyu Road, Hangzhou 310030, Zhejiang, China; School of Life Sciences, Westlake University, 600 Dunyu Road, Hangzhou 310030, Zhejiang, China.

Cell reports methods
|June 26, 2025
PubMed
概括

我们开发了TAS测序 (TAS-seq) 来映射特定细胞区的RNA结构动态. 这种方法揭示了分区特异性RNA调节和细胞RNA异质性,有助于CRISPR-Cas13d系统的优化.

关键词:
CP:细胞生物学 细胞生物学科普:分子生物学RNA 的结构动力学.的gRNA结构和性能.亚细胞腺结构概况分析

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RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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