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短暂与持续的IL-1β驱动炎症3D模型对状纤维细胞的影响

Ana L Castro¹, Esmée van der Vliet², Paulo Pereira³

¹i3S - Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal; INEB - Instituto de Engenharia Biomédica, Universidade do Porto, Porto, Portugal; ICBAS - Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, Portugal.

Acta biomaterialia

|June 26, 2025

概括

椎间盘 (IVD) 中的慢性炎症导致退化. 一个新的3D模型显示,持续的炎症会损害annulus fibrosus (AF) 组织,影响细胞外基质并增加纤维化,这对于理解IVD瘤至关重要.

科学领域:

生物医学工程生物医学工程
细胞生物学细胞生物学
组织工程是组织工程.

背景情况:

慢性炎症是椎间盘 (IVD) 的关键因素,导致细胞外基质 (ECM) 混乱和纤维状环 (AF) 纤维化.
持续炎症环境对AF组织的影响在3D模型中尚未得到充分研究.

研究的目的:

建立和验证3D人源模型,以调查人类AF细胞中慢性炎症的进展.
在3D原I型水凝模型中,比较过渡性和持续性炎症对AF细胞的影响.

主要方法:

在基底和IL-1β诱导的炎症条件下 (暂时或持续),从的IVD中培养出人类AF细胞,在3D原蛋白I型水凝中培养.
为了进行比较,并行进行2D培养.
分析了病理特征,ECM沉积,纤维内素生产和炎症媒介释放.

主要成果:

在持续炎症下的3D模型重述了关键的AF退化特征,包括actin变化和减少I型原沉积.
持续的炎症导致纤维素蛋白的产生增加,并在3D培养物中增加了亲炎性介质.
该模型在炎症条件下体内证明了免疫细胞透的增强.

结论:

在3D原蛋白I型水凝中的持续炎症有效地模拟了与IVD沟相关的AF病理机制.
该模型提供了对ECM和免疫反应持续的炎症影响的见解,这对于研究IVD疾病非常有价值.
这些发现突出了IVD瘤预后的潜在生物标志物和治疗目标.

关键词:

三维文化是3D文化.疾病模型的疾病模型.炎症炎症是一种炎症.椎间盘之间的椎间盘.研究模型研究模型.

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