相关实验视频

Updated: Sep 18, 2025

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Exploring the Regulation of Lipid Droplet Catabolism through Lipophagy

Published on: January 31, 2025

多德二甲基胺氧化物可能诱导溶解体相关的细胞死亡:潜在的脂毒性

Eun-Jung Park¹, Minseok Seo², Wonkyun Jung³

¹Department of Biochemistry and Molecular Biology, College of Medicine, Kyung Hee University, Seoul 02447, Republic of Korea; Human Health and Environmental Toxins Research Center, Kyung Hee University, 02447, Republic of Korea.

Toxicology in vitro : an international journal published in association with BIBRA

|June 26, 2025

概括

家庭用品中的多德二甲基胺氧化物 (DDAO) 通过破坏线粒体和改变脂质代谢,导致细胞死亡. 这表明DDAO可能通过溶酶体通路诱导脂毒性.

科学领域:

细胞生物学细胞生物学
毒理学毒理学毒理学
生物化学生物化学

背景情况:

十二二甲基胺氧化物 (DDAO) 是家用清洁产品中常见的成分.
了解DDAO的细胞毒性机制对于产品安全至关重要.

研究的目的:

研究DDAO在人类支气管上皮细胞中的潜在毒性机制.
为了阐明DDAO暴露的细胞和分子效应.

主要方法:

暴露人类支气管上皮细胞DDAO6小时.
评估细胞活力,细胞阻抗,线粒体功能 (细胞染色体C,ATP生产,线粒体体积) 和细胞内成分.
对细胞因子和矩阵金属蛋白酶 (MMP) 水平的分析.
对LAMP-2蛋白表达和酸性体积的评估.
基因表达分析专注于脂质代谢.

主要成果:

暴露于DDAO降低了细胞活力,并诱导了细胞质真空形成的非亡细胞死亡.
观察到线粒体功能障碍,包括内膜凝结,细胞染色体C增加,ATP产生减少,线粒体体积减少.
DDAO改变了炎症媒介的分泌,增加了IL-6,IL-11,MMP-1和MMP-3.
增加了LAMP-2的表达和扩大的酸性部分被注意到,在40μg/mL时迅速下降.
与脂质代谢相关的基因受到DDAO治疗的显著影响.

结论:

DDAO诱导细胞死亡,可能是通过溶酶体相关的细胞死亡和脂毒性.
线粒体膜的结构损伤是DDAO毒性的关键机制.
lysosomal 膜蛋白可能对DDAO诱导的细胞损伤起着保护作用.

关键词:

消毒剂消毒剂是一种消毒剂.状体类似结构的结构.脂肪毒性脂肪毒性溶酶体细胞死亡 lysosome 是一个溶解体.线粒体中的线粒体.

更多相关视频

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

Published on: July 18, 2019

相关概念视频

Lysosomal Hydrolases

Lysosomal Hydrolases

Lysosomes are the site for the degradation of macromolecules and biological polymers released during membrane trafficking events such as secretory, endocytic, autophagic, and phagocytic pathways. The membrane-enclosed area of the lysosome, called the lumen, contains hydrolytic enzymes active in an acidic environment. These acid hydrolases are functional at a pH between 4.5 and 5 and are involved in cellular processes such as cell signaling, energy metabolism, restoration of the plasma membrane,...

Necrosis

Necrosis

Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...