破坏E3泛素结合酶会产生对疾病的抗性
Xiaoman You1, Jisong Wang1, Guo-Liang Wang2
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Trends in plant science
|June 26, 2025
概括
黄龙 (HLB) 是一种类植物疾病. 研究人员发现,阻断PUB21蛋白稳定MYC2,抑制HLB并使用突变物和人工智能设计的来赋予抵抗力.
科学领域:
- 植物病理学 植物病理学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 黄龙 (HLB) 是一种严重的类病,由细菌Candidatus Liberibacter asiaticus (CLas) 引起.
- 在全球范围内,HLB对类产量产生重大影响,需要有效的疾病管理策略.
- 了解HLB病变的分子机制对于开发耐药类品种至关重要.
研究的目的:
- 调查CLas效应因子SDE5和类植物敏感性因素之间的相互作用.
- 确定增强类植物对黄龙的抗性新目标.
- 探索蛋白质稳定策略对HLB控制的潜力.
主要方法:
- 研究了CLas效应因子SDE5和E3无素结合酶PUB21.21之间的相互作用.
- 使用主导阴性突变体 (PUB21DN) 抑制PUB21活动.
- 使用人工智能 (AI) 设计的抗蛋白解 (APP) 来阻止PUB21的功能.
- 评估了PUB21抑制对MYC2稳定性和HLB抗性的影响.
主要成果:
- 克拉斯的效应因子SDE5针对的敏感因子PUB21.
- PUB21降解MYC2蛋白,从而抑制HLB耐药性.
- 使用PUB21DN或AI设计的APP稳定MYC2.2,抑制PUB21.
- 稳定MYC2给黄龙带来了显著的抵抗力.
结论:
- SDE5-PUB21-MYC2通路是 HLB 耐药性的关键调节模块.
- 准PUB21以稳定MYC2是开发抗HLB果的有希望的战略.
- 人工智能设计的可以为植物的抗病能力提供一种新的方法.
相关概念视频
Regulated Protein Degradation
7.7K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
7.7K
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Receptor Downregulation in MVBs
2.2K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.2K
The Proteasome
1.2K
Eukaryotic cells can degrade proteins through several pathways. One of the most important among these is the ubiquitin-proteasome pathway. It helps the cell eliminate the misfolded, damaged, or unwarranted cytoplasmic proteins in a highly specific manner.
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
In this pathway, the target proteins are first tagged with small proteins called ubiquitin. This involves participation of a series of enzymes including— E1 (ubiquitin-activating enzyme), E2 (ubiquitin-conjugating enzyme), and E3...
1.2K
The Unfolded Protein Response
5.1K
The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
5.1K
Regulation of the Unfolded Protein Response
2.6K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.6K


