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使用3D-RPTEC评估 Puberulic 酸诱导的毒性

Hanwei Peng¹, Etsushi Takahashi², Tomohiro Murakami³

¹Faculty of Pharmaceutical Sciences, Institute of Medical Pharmaceutical and Health Sciences, Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa 920-1192, Japan.

Biological & pharmaceutical bulletin

|June 26, 2025

概括

红酵母大米补充剂中含有的 puberulic 酸会导致细胞损伤. 这项研究证实了它对人类细胞的细胞毒性,强调了与补充剂摄入相关的潜在风险.

科学领域:

腎臟病學 (nephrology) 是一種醫學專業.
毒理学毒理学毒理学
细胞生物学细胞生物学

背景情况:

红酵母大米补充剂与脏近端管道损伤有关.
酸被怀疑是致病剂,但其对人类细胞的影响尚不清楚.

研究的目的:

研究 puberulic 酸对人类初级脏近道管状上皮细胞 (3D-RPTECs) 的细胞毒性作用.
探索有机离子载体 (OAT) 在青春期酸引起的毒性中的作用.

主要方法:

使用了三维培养的初级人类脏近道管状上皮细胞 (3D-RPTECs) 进行细胞毒性测试.
评估球体表面积,线粒体光和细胞内ATP水平在 puberulic 酸暴露后.
研究了probenecid (一种OAT抑制剂) 的作用,并评估了 puberulic 酸对OAT1基质吸收的抑制.

主要成果:

在3D-RPTEC中,暴露于puberulic酸减少了球状体表面积和线粒体光.
观察到细胞内ATP水平的时间依赖性下降,在7天后EC50为24.7μM.
普罗贝尼西德部分减轻了 puberulic 酸的 ATP 降低作用, puberulic 酸抑制了 OAT1 基质的吸收 (IC50 = 5.4 μM).

结论:

酸对人类脏近道管状细胞表现出显著的细胞毒性.
有机离子载体,特别是OAT1,可能参与puberulic酸的毒机制.
这些发现强调了红酵母大米补充剂由于 puberulic 酸的潜在脏风险.

关键词:

造成脏损伤的人.有机离子运输体是有机离子运输体.青春期的青春酸是什么意思红酵母大米补充剂红酵母大米补充剂三维培养初级人类脏近道管状上皮细胞 (3D-RPTEC)

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