相关实验视频
Updated: Sep 18, 2025

07:54
Manipulation of Ploidy in Caenorhabditis elegans
Published on: March 15, 2018
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基因组多样性 X 阳性 乳腺瘤 种类
Chong Chen1,2,3, Taotao Wu1,2,3, Jing Liu1,2,3
1Joint International Research Laboratory of Agriculture and Agri-Product Safety, Institutes of Agricultural Science and Technology Development, Yangzhou University, Yangzhou 225009, China.
Microorganisms
|June 27, 2025
概括
在Myroides细菌中,tigecycline-inactivatingtet(X) 基因的传播是一个全球性的健康威胁. 基因组分析揭示了广泛的X和estT基因多样性和跨物种的转移,突出了抗生素耐药性的风险.
科学领域:
- 微生物学 微生物学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 抗生素耐药性基因的出现和传播,特别是那些对tigecycline等最后手段抗生素产生耐药性的基因,对全球健康构成重大威胁.
- tet (X) 基因家族是tigecycline失活的主要机制,并且越来越多地报告其在各种细菌物种中的存在.
- 格拉姆阴性细菌的一个属Myroides正在成为多药性耐药性 (MDR) 的储存库,但它在tet (X) 基因传播中的作用仍未得到充分探索.
研究的目的:
- 研究Myroides属内的tet (X) 基因的流行,遗传多样性和可转移性.
- 为了描述tet-X阳性Myroides菌株的抗菌素耐药性概况.
- 评估由移动遗传元素介导的X基因的跨物种传播潜力.
主要方法:
- 收集和微生物分析来自中国山东的646个环境和动物样本 (,绵羊,土壤,水) (2021-2024).
- 全基因组测序和生物信息分析九个新分离的MDR Myroides菌株和86个公开可用的 X-阳性 Myroides基因组.
- 遗传学分析以确定进化关系并识别潜在的水平基因转移事件.
主要成果:
- 从样本中分离出9种tet (X) 阳性肌痛菌株,所有这些菌株都表现出多药耐药性 (MDR).
- 基因组分析确定了12种Myroides物种中tet(X) 和宏化物-失活的estT基因的广泛分布,其中包括7种新物种.
- 发现了tet (X) 和estT的八种新型变体,有证据表明IS (CR2) 介导的转子子驱动的tet (X6) 和estT-2的跨物种转移到其他细菌系.
结论:
- 甲状腺类物种携带多种不同的tet (X) 和estT (estT) 基因,有助于扩散tigecycline和macrolide耐药性.
- 已识别的移动遗传元素促进了这些关键耐药性基因的物种间传播.
- 了解像Myroides这样研究不足的细菌中抗生素耐药性的基因组景观,对于开发有效的全球One Health战略至关重要.
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