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带有益生菌潜力的菌酵母菌株:隔离和表征及其对体发酵的影响

Pin Song¹, Xiaoran Yang¹, Manman Hou¹

¹Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing 210095, China.

|June 27, 2025

概括

这项研究发现,将5%的Candida rugosa (NJ-5) 酵母培养物添加到高度饮食中,可以在体外稳定乳头发酵. 这种益生菌酵母改善了挥发性脂肪酸和酸盐水平,但没有改变细菌的多样性.

科学领域:

流体微生物学流体微生物学
动物营养动物营养
益生菌益生菌益生菌

背景情况:

体发酵对于反动物的消化至关重要,特别是在高度饮食中.
酵母补充剂正在被探索,因为它有可能调节乳头功能并改善动物健康.
识别有效的益生菌酵母菌株对于制定有针对性的营养策略至关重要.

研究的目的:

为了隔离和表征乳头酵母菌株的益生菌潜力.
为了评估益生菌酵母Candida rugosa (NJ-5) 对体外起体发酵参数的影响.
为了确定Candida rugosa (NJ-5) 对高度食的反动物的最佳补充水平.

主要方法:

从反体液中分离和识别了59种酵母菌株,随后对益生菌特征进行查.
在体外批量培养发酵,使用70:30的缩料比率,加Candida rugosa (NJ-5) 添加0%,1%,2%和5% (干物质).
皮的pH值,挥发性脂肪酸 (VFA) 和微生物群体结构 (16SrRNA测序) 的分析.

主要成果:

与对照组相比,补充5%的Candida rugosa (NJ-5) 显著减轻了pH值的降低,并增加了总VFA和酸盐.
酵母补充剂没有显著改变牛细菌的α多样性.
有益细菌 (例如,Desulfobacterota,Ruminococcus) 的相对丰富度增加,而一些有害细菌在5%的酵母补充剂下降.

结论:

红菌 (Candida rugosa,NJ-5) 能够有效调节体外粉发酵,特别是在高度养条件下.
5%的干物质补充水平的Candida rugosa (NJ-5) 是最优的,以保持新鲜花发酵的稳定性.
这项研究支持使用Candida rugosa (NJ-5) 作为反动物牲畜的益生菌补充剂,提高消化效率和反动物健康.

关键词:

在Candida rugosa中使用.微生物群落的组成.益生菌酵母是一种益生菌酵母.转动物发酵转动物发酵

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