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从各种水源和人类和动物便中选择的腹致病原体的识别,比较和分析,使用全基因组测序

Arinao Murei¹, Maggy Ndombo Benteke Momba¹

¹Department of Environmental, Water and Earth Sciences, Arcadia Campus, Tshwane University of Technology, 175 Nelson Mandela Avenue, Arcadia, Pretoria 0001, South Africa.

|June 27, 2025

概括

整个基因组测序在水和便样本中确定了腹致病原体,如Shiga毒素产生大肠杆菌. 这突显了公共卫生风险和需要改善水质监测和处理标准的需要.

科学领域:

微生物学微生物学
环境科学环境科学
公共卫生公共卫生

背景情况:

污染的饮用水会导致水源性疾病,如腹和肝炎.
腹致病原体在全球范围内构成重大公共卫生风险.
有效的水质监测对于预防疾病爆发至关重要.

研究的目的:

用全基因组测序 (WGS) 来检测,识别,比较和分析水和便样本中的腹致病原体.
评估特定病原体的流行情况,包括Vibrio cholerae,滋果毒素产生大肠杆菌 (STEC) 和大肠杆菌O157:H7.7.
在不同样本类型中调查这些病原体中毒性和耐药性基因的存在.

主要方法:

全基因组测序 (WGS) 是病原体分析的主要方法.
基于培养的方法,MALDI-TOF质谱和PCR用于最初的病原体识别.
分析包括3168个水样和135个便样本 (人类和动物).

主要成果:

滋果毒素产生大肠杆菌 (STEC) 是WGS检测到的最常见的病原体 (92.9%).
在废水 (60%),处理水 (54.1%) 和地下水 (36.8%) 中,STEC的流行率更高.
在所有样本类型中确定了常见的毒性和耐药性基因,处理过的水和便分离物之间存在重叠.

结论:

水源中的腹致病原体对公众健康构成重大风险.
WGS是一个强大的工具,可以在环境样本中进行全面的病原体分析.
加强水质监测和处理对于减轻与水传播病原体相关的风险至关重要.

关键词:

马尔迪-托夫 (MALDI-TOF) 是一个国家元首.传统的PCR方法.农村社区农村社区水源的水源是水的来源.全基因组测序 (WGS) 是一种全基因组测序.

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