微生物联盟的适应性实验室进化,增强非蛋白质的同化,用于料蛋白质生产
Yi He1, Shilei Wang1,2, Yifan Mi1
1School of Chemical Engineering, Zhengzhou University, Zhengzhou 450001, China.
Microorganisms
|June 27, 2025
概括
可持续的动物料蛋白质是由小麦和非蛋白质 (NPN) 通过微生物发酵生产的. 这一过程提高了蛋白质含量,并为传统料来源提供了有价值的替代品.
科学领域:
- 农业科学 农业科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 全球不断增长的蛋白质需求需要可持续的动物料替代品.
- 基于大豆的料影响人类的粮食安全,推动料行业的创新.
- 利用像小麦一样的农业废物来生产蛋白质对于可持续运营至关重要.
研究的目的:
- 开发一种微生物发酵工艺,用小麦和非蛋白质 (NPN) 来生产高蛋白动物料.
- 通过适应性实验室进化来增强微生物对NPN的耐受性.
- 优化固态发酵 (SSF) 条件,以最大限度地提高小麦中的蛋白质含量.
主要方法:
- 使用NPN和小麦草的微生物联盟的适应性实验室进化.
- 小麦的固态发酵 (SSF) 用一个进化的微生物联盟.
- 优化SSF参数,包括基质类型,注射,添加,湿度,温度和持续时间.
- 扩大优化SSF过程的规模.
主要成果:
- 进化微生物联盟显示,NPN耐受性增加了五倍 (高达5g/L),硫酸的最佳增长.
- 优化SSF条件增加了小麦中的真实蛋白质含量,从2.74%增加到10.42%.
- 扩大规模的SSF工艺 (2.5公斤) 成功生产了具有9.84%真正蛋白质含量的料蛋白质.
结论:
- 小麦和NPN的微生物发酵为生产动物料蛋白提供了一种可持续的方法.
- 适应性实验室进化有效提高微生物对NPN进行发酵的耐受性.
- 这种方法解决了动物料中的蛋白质短缺问题,并增加了农业废物的价值.
更多相关视频
11:51Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
Published on: April 27, 2018
12.0K
06:03Procedure for Adaptive Laboratory Evolution of Microorganisms Using a Chemostat
Published on: September 20, 2016
14.6K
相关概念视频
Inorganic Nitrogen Assimilation
116
Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme...
116
Amino Acid Catabolism
207
Microorganisms rely on proteins as an essential carbon and energy source, particularly in environments with limited polysaccharides or lipids. However, proteins are too large to cross the plasma membrane unaided, necessitating enzymatic degradation. Microbes secrete extracellular proteases and peptidases that hydrolyze proteins into peptides, which can then be transported across the membrane. Once inside the cell, intracellular proteases degrade these peptides into free amino acids, which...
207
Microbial Nutrition
346
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
346
Microorganisms in Agriculture and Food industry
410
Microorganisms play a crucial role in agriculture and the food industry, contributing to soil fertility, crop protection, and food production. Their functions range from nitrogen fixation and biopesticide production to fermentation and food preservation, making them indispensable to sustainable farming and food safety.Role in AgricultureNitrogen-fixing bacteria, such as Rhizobium (symbiotic) and Azotobacter (free-living), convert atmospheric nitrogen into ammonia through biological nitrogen...
410
