塞法兰丁通过氧化应激和CFEM域含蛋白向来抑制Fusarium solani
Yuqing Wang1, Zenghui Yang1, Jingwen Xue1
1School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Zhejiang Provincial Collaborative Innovation Center of Agricultural Biological Resources Biochemical Manufacturing, Hangzhou 310023, China.
Microorganisms
|June 27, 2025
概括
塞法兰丁 (CEP) 显示出作为一种天然抗真菌剂的前景. 这项研究确定了CEP作为Fusarium solani和其他植物病原菌的潜在抑制剂,揭示了其作用机制.
科学领域:
- 自然产品化学 自然产品化学
- 植物病理学 植物病理学
- 计算生物学是一种计算生物学.
背景情况:
- 塞法兰丁 (CEP) 是一种具有已知的抗菌,抗病毒和抗炎性能的双基化物.
- 对于CEP对植物病原菌的抗真菌功效在很大程度上仍未被探索.
- 类物种是重要的农业病原体,在全球造成大量的作物损失.
研究的目的:
- 通过基于机器学习的虚拟查来识别潜在的抗真菌药物对抗Fusarium solani.
- 实验验证塞法兰丁 (CEP) 的抗真菌活性.
- 为了阐明CEP对Fusarium solani的作用机制.
主要方法:
- 机器学习模型 (随机森林,神经网络,支持矢量机器) 用于虚拟选.
- 对多种真菌物种进行CEP的实验测试.
- 分析CEP对真菌生长,化,子发芽和氧化还原平衡的影响.
- 转录组分析和分子对接/动力学模拟以确定目标蛋白质.
主要成果:
- CEP显示出对Fusarium solani,Fusarium proliferatum,Fusarium oxysporum,Alternaria alternata和Botrytis cinerea的显著抑制. 这两种类型的虫有着相对强烈的抗性.
- 在Fusarium solani中,CEP降低了胞和胞发芽,并破坏了其氧化还原平衡.
- 转录组分析揭示了与代谢途径相关的基因表达的改变.
- 分子对接和动力学模拟表明CEP与FsCFEM1蛋白结合,其中关键相互作用涉及THR748和LEU950残留物.
结论:
- 塞法兰丁 (CEP) 是一种强大的,生物基抗真菌剂,具有广泛的活性.
- CEP的抗真菌机制涉及向FsCFEM1蛋白质并破坏细胞氧化还原平衡.
- 这项研究为开发CEP作为新型农业杀菌剂提供了基础.
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