Limosilactobacillus reuteri M4-100 在肠道上皮模型中减轻了大肠杆菌菌株HMLN-1的致病性,并调节了宿主细胞基因表达
Behnoush Asgari1, Georgia Bradford1, Eva Hatje2
1Centre for Bioinnovation, and School of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore, QLD 4558, Australia.
Microorganisms
|June 27, 2025
概括
益生菌Limosilactobacillus reuteri M4-100显著抑制大肠杆菌HMLN-1的转位和粘附到肠道细胞. 用这种益生菌进行预治疗显示出明显的预防作用,防止大肠杆菌入侵.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
背景情况:
- 益生菌越来越多地被认为具有健康益处.
- 了解肠道中的益生菌和病原细菌之间的相互作用对于宿主健康至关重要.
- 大肠杆菌HMLN-1是一种能够转移肠道屏障的菌株.
研究的目的:
- 调查益生菌Limosilactobacillus reuteri M4-100和转位大肠杆菌HMLN-1菌株之间的相互作用.
- 阐明主体对这些细菌相互作用反应背后的分子机制.
- 评估L. reuteri M4-100在预防大肠杆菌HMLN-1粘附,入侵和转移方面的疗效.
主要方法:
- 肠道上皮的共同培养模型 (Caco-2:HT29-MTX细胞).
- 对大肠杆菌HMLN-1的共同培养暴露,使用电子显微镜进行转位途径分析.
- 与L. reuteri M4-100和E. coli HMLN-1进行同期和预注射实验.
- 差异基因表达分析以确定宿主细胞反应.
主要成果:
- L. reuteri M4-100显著降低了大肠杆菌HMLN-1对肠道细胞共同培养的附着性 (p < 0.0001).
- 使用L. reuteri M4-100的预注射显著减少了大肠杆菌HMLN-1的入侵 (p < 0.0001).
- 在同注射和注射前的环境中,L. reuteri M4-100显著抑制了大肠杆菌HMLN-1转位 (p < 0.0001).
- 不同的基因表达揭示了对单个细菌菌株的关键宿主反应.
结论:
- Limosilactobacillus reuteri M4-100有效地减少或抑制大肠杆菌HMLN-1与肠道上皮的相互作用.
- 益生菌菌株L. reuteri M4-100具有显著的预防作用,特别是当在暴露于病原体之前服用时.
- 这些发现支持L. reuteri M4-100的潜在用途,作为维持肠道屏障完整性的益生菌干预.
关键词:
埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.利莫西拉克托巴西勒斯 (Limosilactobacillus reuteri) 是一种植物.不同的基因表达.肠道上皮质 肠道上皮质转移转移是指转移的转移.更多相关视频
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