相关实验视频
Updated: Sep 18, 2025

13:25
Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish
Published on: May 15, 2021
5.9K
达沙替尼和奎尔塞丁组合在急性叶酸诱导的实验性病症中增加了损伤
Antonio Battaglia-Vieni1,2, Vanessa Marchant1,2, Lucia Tejedor-Santamaria1,2
1Molecular and Cellular Biology in Renal and Vascular Pathology Laboratory, Department of Medicine, IIS-Fundación Jiménez Díaz, Universidad Autónoma de Madrid, Avda. Reyes Católicos, 2, 28040 Madrid, Spain.
Pharmaceuticals (Basel, Switzerland)
|June 27, 2025
概括
达沙替尼加奎尔塞丁 (D&Q) 的老化药物组合在小鼠中没有防止急性损伤 (AKI). D&Q治疗加剧了一些有害反应,这表明在AKI中对临床翻译要谨慎.
科学领域:
- 腎臟病學 (nephrology) 是一種醫學專業.
- 老年学是一门学科.
- 细胞生物学 细胞生物学
背景情况:
- 急性损伤 (AKI) 是一个重大的临床挑战,死亡率高,进展为慢性病 (CKD),特别是在老年人中.
- 细胞衰老与AKI,CKD和衰老有关,是潜在的治疗标.
- 抗衰老药物,如达沙替尼加奎尔丁 (D&Q),在实验性CKD等疾病中表现有前途,但它们在AKI中的疗效尚不清楚.
研究的目的:
- 在急性损伤的小鼠模型中研究老化药物组合达沙替尼加奎尔 (D&Q) 的治疗潜力.
- 在AKI的背景下,评估D&Q对功能,损伤生物标志物,细胞衰老标志物和抗衰老因素的影响.
主要方法:
- 一个叶酸诱导的毒性 (FAN-AKI) 鼠标模型被用于研究AKI.
- 在诱导AKI之前,小鼠先用D&Q组合进行预治疗.
- 评估了功能 (血清肌素,BUN),损伤的基因表达和衰老标志物,p21阳性衰老细胞和Klotho水平.
主要成果:
- 在FAN-AKI急性阶段,D&Q预治疗未能预防功能障碍,如血清肌素和BUN水平所示.
- D&Q治疗显著增加了损伤生物标志物 (Lcn2,Havcr1),p21和与衰老相关的分泌表现型基因的表达.
- 衰老的p21阳性细胞的数量和Klotho的下调,在受伤脏的D&Q治疗中保持不变.
结论:
- 老化药物组合D&Q在叶酸诱导的急性损伤中没有保护作用.
- 在FAN-AKI模型中,D&Q治疗加剧了某些有害反应,突出了潜在的风险.
- 这些发现表明,关于D&Q在AKI治疗中的临床转化,需要谨慎.
相关概念视频
Acute Kidney Injury IV: Diagnostic Studies and Prevention
59
Accurate diagnosis and effective prevention are critical in managing Acute Kidney Injury (AKI), which is linked to high mortality rates ranging from 10% to 80%. Timely recognition of at-risk patients and careful monitoring can significantly reduce the likelihood of kidney damage.Diagnostic Assessments:The diagnostic process starts with a comprehensive medical history to identify prerenal, intrarenal, and postrenal causes.Prerenal causes, such as dehydration, hypotension, or blood loss, should...
59
Renal Failure: Dose Adjustments
166
In patients with renal impairment, drugs undergo significant changes in their pharmacokinetics, which require dosage adjustments to ensure safe and effective therapy.
Reduced renal clearance and elimination rate are common outcomes of renal impairment. These alterations lead to a prolonged elimination half-life and an altered apparent volume of distribution for drugs. As a result, dosage adjustments are typically necessary to maintain optimal drug levels in the body.
However, dosage adjustments...
Reduced renal clearance and elimination rate are common outcomes of renal impairment. These alterations lead to a prolonged elimination half-life and an altered apparent volume of distribution for drugs. As a result, dosage adjustments are typically necessary to maintain optimal drug levels in the body.
However, dosage adjustments...
166
Acute Kidney Injury II: Pathophysiology
97
Acute kidney injury (AKI) causes are categorized into three primary categories based on the location of the injury: prerenal, intrarenal (or intrinsic), and postrenal causes. This classification guides clinical management and illustrates how different pathways can impair kidney function.Etiology and Pathophysiology of Acute Kidney Injury1. Prerenal causesEtiology: Prerenal Acute Kidney Injury, the most common type, occurs when reduced blood flow to the kidneys decreases filtration capacity...
97
Acute Kidney Injury V: Interprofessional Care
49
Acute Kidney Injury (AKI) requires a collaborative healthcare approach to restore renal function and prevent complications. Essential management strategies involve monitoring fluid and electrolyte balance, adjusting medications, initiating dialysis when necessary, and providing nutritional support.Fluid and Electrolyte ManagementFluid Monitoring: Regularly monitoring body weight, central venous pressure, and urine output helps detect fluid imbalances early. Patient intake and output are...
49
Acute Kidney Injury I: Introduction
82
Introduction:Acute Kidney Injury (AKI) describes a swift decrease in kidney function occurring over hours to days, characterized by the kidneys' failure to remove waste products from the bloodstream. This leads to dangerous complications like metabolic acidosis, fluid overload, and electrolyte imbalances, such as hyperkalemia, which can cause life-threatening arrhythmias. AKI is common in both hospital and outpatient settings, often triggered by dehydration, sepsis, or exposure to nephrotoxic...
82
Drugs that Destabilize Microtubules
2.0K
Microtubules are dynamic structures and can be regulated by microtubule targeting agents (MTAs). Microtubule destabilizing drugs are a class of MTAs that destabilize and prevent microtubules' polymerization. Both natural and synthetic chemicals can be found under this class of drugs. Vincristine and vinblastine, two vinca alkaloids, and colchicine were among the first to be discovered. These drugs can affect cells in various ways, either by inducing a change in cell morphology, preventing...
2.0K

