通过使用干卡运输核酸样本来促进跨境病毒测序.
Lili Wang1,2, Qikai Yin1, Alie Brima Tia3
1National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Viruses
|June 27, 2025
概括
弗林德斯技术协会 (FTA) 卡片使得在资源有限的环境中,从放大核酸样本中进行病毒基因组测序. 这种方法提高了在欠发达地区的传染病监测和疫情应对能力.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 公共卫生 公共卫生
背景情况:
- 发展中国家往往缺乏用于传染病监测的先进测序基础设施.
- 在资源有限的地区,需要一种新的核酸样本运输方法来进行病毒基因组测序.
研究的目的:
- 评估弗林德斯技术协会 (FTA) 卡片在传输SARS-CoV-2扩增全基因组DNA方面的有效性.
- 评估在资源有限的环境中使用FTA卡运输的样本进行病毒基因组测序的可行性.
主要方法:
- 在塞拉利昂收集了120个SARS-CoV-2阳性鼻口腔拭子样本.
- 核酸提取和全基因组放大是在本地进行的.
- 放大DNA应用于FTA Elute卡片,用于室温运输和随后的化和测序.
主要成果:
- 通过FTA卡方法,AMPLCON恢复率达到9.6%,足以进行病毒基因组测序.
- 获得了86个 (71.7%) 高质量的SARS-CoV-2基因组序列,其中大多数超过100X的深度.
- 确定了三角形,欧米克朗和B.1血统的同时流通;Ct ≤27的样本产生了有效的序列 (73.6%).
结论:
- 在FTA卡上运输放大核酸样本有助于在资源有限的地区进行病毒基因组测序.
- 这种方法可以加强本地病毒监测和疫情应对.
- 进一步优化可能会提高序列恢复率,增加在欠发达地区的序列可访问性.
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