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一种基于聚氨酸的RBD粘膜疫苗诱导了小鼠的强烈抗体反应

Huifang Xu¹, Han Wang¹, Peng Sun^1,2

¹Laboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, 20 Dongdajie Street, Fengtai District, Beijing 100071, China.

|June 27, 2025

概括

开发有效的粘膜疫苗至关重要. 这项研究将SARS-CoV-2尖端受体结合域 (RBD) 设计成聚氨酸,并将其与霍乱毒素B亚单元 (CTB) 结合起来,以在小鼠中实现强大,持久的免疫反应.

科学领域:

疫苗学疫苗学疫苗学
免疫学免疫学免疫学
蛋白质工程是指蛋白质工程.

背景情况:

随着COVID-19的爆发,人们越来越需要先进的粘膜疫苗策略.
目前的疫苗方法需要加强有效的粘膜免疫力.

研究的目的:

开发针对SARS-CoV-2尖端蛋白的受体结合域 (RBD) 的新型粘膜疫苗.
评估工程RBD配方与聚氨酸和霍乱毒素B子单元 (CTB) 的免疫性.

主要方法:

用聚氨酸 (K10和K5) 设计了RBD,以增加正电荷.
在BALB/c小鼠中通过肺路径给药的工程RBD配方与CTB.
评估了血清和支气管支气管洗液中的抗原特异性抗体反应.

主要成果:

肺部给药K10-RBD与CTB诱导了强大的中和抗体标位.
在第三次免疫接种后的六个月内,观察到持续高血清IgG抗体标位 (>10^4).
与CTB结合的K5-RBD显著提高了血清IgG和IgA抗体水平.

结论:

聚氨酸修改RBD,当与CTB结合时,有效地刺激粘膜和幽默免疫力.
这些发现为设计针对SARS-CoV-2的亚单元粘膜疫苗提供了基础.
工程设计的RBD-CTB配方显示了长期免疫诱导的潜力.

关键词:

在RBD中,RBD是RBD.这就是SARS-CoV-2病毒.这是一种粘膜疫苗.聚氨酸聚氨酸是什么一个子单位的疫苗.

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