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在羊群中评估Orf病毒载体基础的疫苗输送平台的免疫性

Sean R Wattegedera¹, Jackie Thomson¹, Lesley Coulter¹

¹Moredun Research Institute, Pentlands Science Park, Penicuik EH26 0PZ, UK.

|June 27, 2025

概括

一种经过修改的Orf病毒载体疫苗有效地刺激了针对绵羊主要外膜蛋白 (MOMP) 抗原的抗体和细胞免疫反应,显示出对细菌疾病控制的希望.

科学领域:

兽医免疫学兽医免疫学
疫苗的研发工作正在进行中.
病毒载体技术的病毒载体技术.

背景情况:

基于病毒的疫苗载体被确立为病毒感染,并显示出潜在的细菌疾病.
主要的外膜蛋白 (MOMP) 抗原是对像羊类动物性流产这样的甲状腺炎感染的保护.
细胞干扰素- (IFN-γ) 生产对于控制Chlamydiae至关重要.

研究的目的:

为了比较修改后的Orf病毒和羊 maedi visna病毒载体,用于在羊中传递Chlamydia abortus ompA抗原.
评估在羊群中基于修改的Orf病毒疫苗 (mORFV-ompA) 的免疫性,评估活体和非活化形式.
为了确定mORFV-ompA疫苗引起的细胞和幽默免疫反应.

主要方法:

绵羊通过肌肉免疫接种活的mORFV-ompA (n=10),非活化的mORFV-ompA (n=10),或作为未接种疫苗的对照 (n=10).
对抗MOMP抗体 (免疫球蛋白G) 进行了血清学评估.
细胞召回反应,包括IFN-γ和互白素 (IL) -17A分泌,通过淋巴细胞刺激试验在外周血液单核细胞中测量.

主要成果:

活体和非活化mORFV-ompA疫苗都诱导了抗MOMP免疫球蛋白G抗体.
在两个疫苗组中都检测到抗原特异性细胞反应,其特征是IFN-γ和IL-17A分泌.
没有观察到对Orf病毒疫苗平台的抗体反应,IL-10和IL-4的产生可以忽略不计.

结论:

活体和无活化的mORFV-ompA疫苗都可以作为绵羊免疫的平台技术.
这种方法解决了对无助剂诱导幽默和细胞反应的兽医疫苗的需求.
成功使用MOMP抗原表明了开发针对细菌疾病的疫苗的潜力.

关键词:

克拉米迪亚流产的流产.幽默和细胞免疫学分析免疫性免疫性免疫性主要的外膜蛋白质主要外膜蛋白质.疫苗开发平台疫苗开发平台基于病毒的疫苗疫苗.

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