在黑士兵 (Hermetia illucens) 中使用基因型测序来确定亲属关系
Guyllaume Dufresne1, Catherine Bolduc2, Christopher Warburton2
1Department of Animal Science, Faculty of Agricultural and Food Sciences, Université Laval, Quebec, QC, Canada.
Frontiers in genetics
|June 27, 2025
概括
这项研究开发了单核酸多态 (SNP) 面板,用于黑兵 (BSF) 的亲属关系分配. 这些SNP面板能够准确的基因跟踪,这对于优化昆虫的繁殖计划至关重要,因为食品和料行业.
科学领域:
- 动物遗传学动物遗传学
- 昆虫生物技术 昆虫生物技术
- 量化遗传学 量化遗传学
背景情况:
- 基因选择是改善黑兵 (BSF) 经济重要特征的关键.
- 精确的血统跟踪对于遗传进步和在BSF繁殖计划中保持遗传多样性至关重要.
- 目前的血统追踪方法对于像BSF这样的大规模养的昆虫来说是不可行的.
研究的目的:
- 为了在BSF中确定亲属性赋值 (PA) 的信息性单核酸多态 (SNP).
- 用不同的SNP面板密度来评估PA的准确性和可靠性.
- 支持为BSF开发有效的育种计划.
主要方法:
- 用基因型测序 (GBS) 来识别商业和实验室BSF殖民地中的SNP.
- 实验种群建立在三代之间,每个殖民地有12个家庭.
- 在交配对和幼虫 (n=288) 上进行了DNA测序,随后使用基于概率的方法进行了SNP调用,过和PA.
主要成果:
- 在所有测试的SNP子集和近亲繁殖率中,亲属关系分配的准确性达到了100%.
- 分配信心 (LOD得分) 随着SNP的减少而显著下降,但不受内生繁殖的影响.
- 高信任亲属关系分配需要192个SNP单个父母和118-192个SNP的父母对.
结论:
- 识别的SNP对于在BSF中创建低密度面板以进行基于血统的选择是有价值的.
- 这些SNP资源将有助于管理遗传多样性并推进BSF繁殖计划.
- 开发的SNP小组为大规模养昆虫的遗传管理提供了可行的解决方案.
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