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Updated: Sep 17, 2025

A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue
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A Simplified and Efficient Method to Isolate Primary Human Keratinocytes from Adult Skin Tissue

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从人类皮肤样本中优化了蛋白质提取协议

Ana Paula Carvalho Reis1, Giovanna Azevedo Celestrino1, Talita Souza Siqueira2

  • 1Laboratory of Medical Mycology LIM-53, Division of Clinical Dermatology, Instituto de Medicina Tropical, Hospital das Clínicas, Faculty of Medicine, Universidade de São Paulo, Avenida Doutor Enéas de Carvalho Aguiar, 470, São Paulo, SP, 05403-000, Brazil.

Biology methods & protocols
|June 27, 2025
PubMed
概括

研究人员优化了人类皮肤蛋白质提取方法,识别了约6000种皮肤蛋白. 这一进步有助于了解皮肤生物学和疾病生物标志物的发现.

关键词:
蛋白质的提取 蛋白质的提取质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量蛋白质组学 蛋白质组学协议 协议 协议 协议 协议皮肤 皮肤 皮肤

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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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