明亮和水可分散的膜探针能够可视化活小鼠光散射组织中的细胞形态和动态
Takumi Uemura1, Ryosuke Kawakami2, Hitomi Seki1
1Research and Education Faculty, Multidisciplinary Science Cluster, Interdisciplinary Science Unit, Kochi University 2-5-1 Akebono-cho, Kochi-shi Kochi 780-8520 Japan y.niko@kochi-u.ac.jp.
Chemical science
|June 27, 2025
概括
研究人员开发了一种新的光探针dSQ12AQ,用于可视化活体动物细胞结构. 这种明亮的,在水中分散的探针允许在体内成像完整组织中的细胞形态和动态.
科学领域:
- 生物物理学的生物物理.
- 细胞生物学 细胞生物学
- 医疗成像医学成像
背景情况:
- 光等离子膜探针对于研究细胞结构和动态至关重要.
- 目前的探针仅限于培养细胞或固定组织,阻碍了体内应用.
- 在完整的组织中可视化细胞形态仍然是一个重大挑战.
研究的目的:
- 开发和评估一种新的,明亮的,可在水中分散的光探针,用于体内细胞成像.
- 评估探头在完整的组织和器官中可视化整个细胞形态的能力.
- 为了证明探测器在活体动物细胞过程的动态成像中的实用性.
主要方法:
- 合成一种新的基于四角蛋白的膜探针,dSQ12AQ,具有离子基组,用于水分散性.
- 在活小鼠中静脉注射dSQ12AQ.
- 使用双光子显微镜进行体内成像.
- 在骨髓血管和周血管区域观察细胞形态.
- 评估探针外流和可视化皮肤组织中的细胞.
主要成果:
- dSQ12AQ在高度下表现出高水分散性和稳定性.
- 静脉注射使得小鼠的全细胞形态在体内清晰可视化.
- 在骨髓血管系统中实现了血液细胞行为的动态成像.
- 探测器成功地从血管中脱离了血管,可视化了周围血管和皮肤区域的细胞.
- 皮肤中的角质细胞,纤维细胞和汗道细胞的可视化成功.
结论:
- dSQ12AQ是一种多功能且有效的光探针,用于体内细胞成像.
- 探测器有助于在完整的组织和器官中可视化细胞形态和动态.
- dSQ12AQ具有重要的潜力,可以在体内研究细胞过程和疾病.
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