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Updated: Sep 17, 2025

Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80
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Protein Purification Technique that Allows Detection of Sumoylation and Ubiquitination of Budding Yeast Kinetochore Proteins Ndc10 and Ndc80

Published on: May 3, 2015

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一个协调的激酶和酸酶网络调节了Stu2对酵母激酶的招募

Michael G Stewart1, Joseph S Carrier1, Jacob A Zahm2

  • 1Department of Biochemistry, University of Utah School of Medicine, Salt Lake City, UT, USA.

The Journal of cell biology
|June 27, 2025
PubMed
概括

在T866,Stu2微管聚合酶局部化受Polo样酶1 (PLK1/Cdc5) 酸化的调节,控制了亚纳相螺旋的进展. 这种酸化与PP2A/Cdc55酸酶相反,使得Stu2能够及时重新分配细胞分裂.

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

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