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通过DART-MS/MS对布塔的高通量定量,加上微固相提取和双碎片,以提高选择性和灵敏度

Huaqiang Li¹, Feifei Wu², Yuxian Ding¹

¹Guangdong Provincial Key Laboratory for Green Agricultural Production and Intelligent Equipment, College of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Maoming, 525000, Guangdong, China.

Analytical and bioanalytical chemistry

|June 27, 2025

概括

实时联质谱法 (DART-MS/MS) 试验中的一种新的直接分析快速量化了布塔. 这种使用微固相提取 (μ-SPE) 的方法为环境和毒理学分析提供了更快,更绿色的替代方案.

科学领域:

分析化学分析化学
环境科学环境科学
毒理学毒理学毒理学

背景情况:

丁是一种广泛使用的除草剂,需要敏感的量化方法.
传统的布塔分析技术可能耗时,并产生大量的浪费.
需要快速,高效和环保的测定方法来进行布塔分析.

研究的目的:

开发和验证一种用于量化布塔的新型快速分析试验.
评估MCF-7细胞中布塔的细胞吸收动力学.
为了证明DART-MS/MS对环境和毒理学评估的有用性.

主要方法:

在实时联质谱法 (DART-MS/MS) 试验中开发无染色学直接分析.
纳入微固相提取 (μ-SPE) 作为内部标准,用于用乙化预处理样品.
使用特定离子转换对布塔和乙进行量化.

主要成果:

在DART-MS/MS试验中,实现了高分析吞吐量 (0.4分钟/样本),在0.5-100 ng/mL上具有优异的线性 (R2>0.995).
方法验证显示了良好的精度 (<13.1%) 和准确性 (-6.00%至11.0%).
该试验成功地用于评估MCF-7细胞中布塔细胞吸收动力学.

结论:

DART-MS/MS测定提供了一种快速,灵敏和可持续的方法来定量布塔.
与传统方法相比,这种方法减少了环境影响和分析时间.
这些发现突显了布塔暴露的潜在安全问题以及该测试在生态毒理学研究中的价值.

关键词:

甲基酸的使用方法细胞吸收的时间在DART-MS/MS中使用.微固体相提取的微固体相提取方法量化量化量化量化

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