两种常用和有限使用的化剂对Lucilia sericata卵表面灭菌的作用
Nevra Polat1, Salih Mollahaliloglu2, Murat Koc3
1Department of Traditional Complementary and Integrative Medicine, Institute of Public Health, Ankara Yıldırım Beyazıt University, Ankara, Turkey. nevrapolat@aybu.edu.tr.
治疗需要无菌的蛋. 这项研究发现,在0.05%的低化中浸泡1分钟有效消毒Lucilia sericata蛋,确保了治疗应用的高存活率.
科学领域:
- 生物医学研究的研究.
- 医学昆虫学 医学昆虫学
- 创新的伤口护理创新.
背景情况:
- 疗法 (MT) 利用无菌的幼虫治疗无法愈合的伤口,为手术和长时间的消毒治疗提供了替代方案.
- 有效的MT取决于无菌技术,以防止伤口感染,因为蛋可能被细菌污染.
- 在MT中常见的Calliphoridae家族的,产卵需要在使用前进行表面杀菌.
研究的目的:
- 为了比较低酸盐 (NaOCl) 和Lysol表面消毒剂在杀菌Lucilia sericata蛋中的有效性.
- 评估不同度和应用时间对卵子活力和死亡率的影响.
- 确定用于治疗的Lucilia sericata蛋的最佳绝育方案.
主要方法:
- 卢西利亚虫蛋经过使用NaOCl (S1) 和Lysol (S2) 在不同度 (0.05-3%) 和暴露时间 (1-4分钟) 的消毒.
- 绝育的有效性通过在实验室中对三角性大豆 (TSA) 的测试来评估,以检测细菌和真菌的生长.
- 在每个绝育条件下记录了卵子存活率和死亡率.
主要成果:
- NaOCl和lysol都有效地杀菌了蛋,在经过处理的蛋上没有观察到细菌或真菌生长.
- 确定了最优的绝育方案,即在0.05%的NaOCl溶液中浸泡1分钟.
- 该方案实现了有效的绝育,卵子存活率高达85.12%,死亡率低达14.88%.
结论:
- 建议在0.05%的低化溶液中浸泡1分钟,以有效绝育Lucilia sericata的蛋.
- 这种方法平衡了有效的细菌消除与高卵子活力,这对于可靠的虫治疗至关重要.
- 这些发现有助于建立用于治疗准备的标准化无菌协议.
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