相关实验视频

Updated: Sep 17, 2025

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art

Published on: May 28, 2019

基因组集成磁带交换:将Saccharomyces cerevisiae的模块化带到菌株水平

Pietro Butti¹, Francesco Bellusci¹, Elisa Brambilla¹

¹Department of Biotechnology and Biosciences, University of Milano Bicocca, Piazza della Scienza 2, 20126 Milan, Italy.

FEMS yeast research

|June 27, 2025

概括

这项研究引入了一个基于CRISPR的系统,可以轻松地交换或删除Saccharomyces cerevisiae中的基因表达盒. 这提高了微生物细胞工厂建设的模块化,简化了菌株工程.

科学领域:

合成生物学合成生物学
分子生物学分子生物学
基因工程是一种基因工程.

背景情况:

对于Saccharomyces cerevisiae,现有的合成生物学工具包缺乏用于修改集成遗传磁带的标准化方法.
这种限制阻碍了微生物细胞工厂建设的模块化,使其顺序和刚性.

研究的目的:

开发一种标准化系统,以CRISPR为媒介,在Saccharomyces cerevisiae中进行预集成基因磁带的交换或移除.
增强菌株工程模块化,简化微生物细胞工厂的修改.

主要方法:

开发一个系统,在表达卡塞特上标记特定的条形码.
使用CRISPR核酶 (Cas9和Cas12a) 针对这些条形码进行磁带切割和更换.
与Easy-MISE工具包的集成,以实现实际应用.

主要成果:

证明了光蛋白表达卡塞特的有效 (∼90%) 交换.
通过使用标准化,单步骤的过程,成功地移除和替换集成磁带.
通过PCR和流细胞计验证.

结论:

开发的系统有效地为Saccharomyces cerevisiae的菌株水平带来了模块化.
这种方法大大减少了修改现有微生物菌株所需的步骤.
促进微生物细胞工厂的更灵活,更高效的建设.

关键词:

这种植物是Saccharomyces cerevisiae.克里斯普尔是什么意思?克里斯普尔是什么意思?易于错过的工具包磁带交换磁带交换磁带交换基因组编辑基因组编辑这是模块化的模块化.

更多相关视频

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

The Green Monster Process for the Generation of Yeast Strains Carrying Multiple Gene Deletions

Published on: December 15, 2012

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Rapid Assembly of Multi-Gene Constructs using Modular Golden Gate Cloning

Published on: February 5, 2021

相关概念视频

Protein Complexes with Interchangeable Parts

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...