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Updated: Sep 17, 2025

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Temporal Analysis of the Nuclear-to-cytoplasmic Translocation of a Herpes Simplex Virus 1 Protein by Immunofluorescent Confocal Microscopy

Published on: November 4, 2018

在HSV-1核聚合物中对核糖体生物发生因子的隔离,通过空间分辨的热谱分析揭示出来

Peter J Metzger¹, Tavis J Reed^1,2,3, Krystal K Lum¹

¹Department of Molecular Biology, Princeton University, 119 Lewis Thomas Laboratory, Princeton, NJ 08544, USA.

Science advances

|June 27, 2025

概括

简单疹病毒1型 (HSV-1) 感染导致宿主细胞形成病毒诱导的伴侣丰富 (VICE) 域. 这些域破坏了核糖体生物发生,损害了蛋白质的产生,并帮助病毒的复制.

科学领域:

病毒学病毒学
细胞生物学细胞生物学
分子生物学分子生物学

背景情况:

病毒操纵宿主细胞的分离,以便复制.
简单疹病毒1型 (HSV-1) 改变宿主蛋白质定位以控制免疫反应,基因表达和蛋白质合成.
了解感染期间的动态蛋白质-蛋白质相互作用 (PPI) 是至关重要的.

研究的目的:

在HSV-1感染期间空间解析动态PPI网络.
研究分区特异性蛋白质复合体的形成和功能.
为了描述病毒诱导的陪伴者增强 (VICE) 域.

主要方法:

核/细胞质分离与热近距离聚合分析 (N/C-TPCA) 的整合.
在VICE领域内监测蛋白质和RNA成分.
在感染期间评估核糖体活动.

主要成果:

在HSV-1感染期间,N/C-TPCA提供了高分辨率,隔间特定的PPI地图.
比预期更广泛的宿主陪伴者被招募到核中,形成VICE域.
VICE 域隔离了核糖体生物发生因子,导致核糖体供应的全球减少和翻译受损.

结论:

感染HSV-1诱导核VICE域的形成,这是翻译重塑的关键网站.
VICE 域隔离了关键的核糖体生物发生因子,导致核糖体生产中的整个宿主缺陷.
N/C-TPCA是一种强大的技术,用于研究特定细胞区内的动态生物过程和蛋白质相互作用.

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