Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Catalytically Perfect Enzymes01:07

Catalytically Perfect Enzymes

4.1K
The theory of catalytically perfect enzymes was first proposed by W.J. Albery and J. R. Knowles in 1976. These enzymes catalyze biochemical reactions at high-speed. Their catalytic efficiency values range from 108-109 M-1s-1. These enzymes are also called 'diffusion-controlled' as the only rate-limiting step in the catalysis is that of the substrate diffusion into the active site. Examples include triose phosphate isomerase, fumarase, and superoxide dismutase.
 
Most enzymes...
4.1K
Introduction to Mechanisms of Enzyme Catalysis01:13

Introduction to Mechanisms of Enzyme Catalysis

8.9K
For many years, scientists thought that enzyme-substrate binding took place in a simple "lock-and-key" fashion. This model stated that the enzyme and substrate fit together perfectly in one instantaneous step. However, current research supports a more refined view scientists call induced fit. The induced-fit model expands upon the lock-and-key model by describing a more dynamic interaction between enzyme and substrate. As the enzyme and substrate come together, their interaction causes...
8.9K
Enzymes02:34

Enzymes

82.8K
Inside living organisms, enzymes act as catalysts for many biochemical reactions involved in cellular metabolism. The role of enzymes is to reduce the activation energies of biochemical reactions by forming complexes with its substrates. The lowering of activation energies favor an increase in the rates of biochemical reactions.
Enzyme deficiencies can often translate into life-threatening diseases. For example, a genetic abnormality resulting in the deficiency of the enzyme G6PD...
82.8K
Cofactors and Coenzymes01:24

Cofactors and Coenzymes

11.3K
Enzymes are proteins made of amino acids. The functional group of each constituent amino acid catalyzes a wide variety of chemical reactions via ionic interactions or acid-base reactions. However, amino acids cannot catalyze oxidation-reduction and group transfer reactions and need to be aided by non-protein components called cofactors. Cofactors are also referred to as the chemical teeth of an enzyme.
Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
11.3K
Introduction to Enzymes01:22

Introduction to Enzymes

20.0K
The use of enzymes by humans dates to 7000 BCE. Humans first used enzymes to ferment sugars and produce alcohol without knowing that this was an enzyme-catalyzed reaction. Wilhelm Kuhne coined the term 'enzyme' in 1877 from the Greek words ‘en’ meaning ‘in’ or ‘within’ and ‘zyme’ meaning ‘yeast.’
Most enzymes are proteins that speed up biochemical reactions without being consumed. Enzymes contain one or more active sites that...
20.0K
Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

20.9K
Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
20.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Core-to-core overlap enables efficient interchain charge transport beyond crystalline domains in a conjugated polymer: high fill factors in thick organic photovoltaic cells.

Chemical science·2026
Same author

Gas-loading system compatible with ultrafast magic-angle spinning for solid-state nuclear magnetic resonance in gas atmospheres.

Chemical communications (Cambridge, England)·2026
Same author

Chirality Transfer from Covalent Organic Framework Nanotubes to Covalent Organic Framework Films via Chirality Induction Crystallization.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Semirenewable Polyamides Containing Disulfide Bonds: Synthesis, Degradation, Self-Healing, and Triboelectric Properties.

Macromolecules·2026
Same author

Tuning of Electron-Donating Metal-Organic Frameworks toward High-Performance Triboelectric Nanogenerators for Self-Powered Shear Sensing.

ACS applied materials & interfaces·2026
Same author

Robust triboelectric energy harvesters engineered from electrochemically deposited films of HKUST-1 polycrystals.

Communications chemistry·2026

相关实验视频

Updated: Sep 17, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

17.5K

用于生物催化剂的共沉积酶封装的共价有机框架

Satyadip Paul1, Mani Gupta2, Shayan Karak1

  • 1Department of Chemical Sciences, Indian Institute of Science Education and Research, Mohanpur, Kolkata 741246, India.

Journal of the American Chemical Society
|June 27, 2025
PubMed
概括

我们开发了一种单合成方法, 将酶封装在共价有机框架 (COF) 中, 提高它们的稳定性和可重复使用性. 这种方法在恶劣条件下提高了酶性能,使生物催化剂的应用范围更广.

更多相关视频

Microfluidic-based Synthesis of Covalent Organic Frameworks COFs: A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface
08:42

Microfluidic-based Synthesis of Covalent Organic Frameworks COFs: A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface

Published on: July 10, 2017

13.6K
Preparation of Functional Silica Using a Bioinspired Method
08:04

Preparation of Functional Silica Using a Bioinspired Method

Published on: August 1, 2018

17.4K

相关实验视频

Last Updated: Sep 17, 2025

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability
09:27

Immobilization of Multi-biocatalysts in Alginate Beads for Cofactor Regeneration and Improved Reusability

Published on: April 22, 2016

17.5K
Microfluidic-based Synthesis of Covalent Organic Frameworks COFs: A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface
08:42

Microfluidic-based Synthesis of Covalent Organic Frameworks COFs: A Tool for Continuous Production of COF Fibers and Direct Printing on a Surface

Published on: July 10, 2017

13.6K
Preparation of Functional Silica Using a Bioinspired Method
08:04

Preparation of Functional Silica Using a Bioinspired Method

Published on: August 1, 2018

17.4K

科学领域:

  • 生物催化
  • 材料科学
  • 纳米技术

背景情况:

  • 酶是有效的生物催化剂,但在恶劣的工业条件下表现出有限的稳定性.
  • 开发强大的酶固定化策略对于扩大其实际应用至关重要.
  • 由于它们的可调结构和高表面积,共价有机框架 (COF) 为生物分子封装提供了一个有前途的平台.

研究的目的:

  • 开发一种易于用酶封装的COF的水性合成方法.
  • 研究封装酶和COF矩阵之间的相互作用.
  • 评估封装酶的增强稳定性和可重复使用性.

主要方法:

  • 包括β-葡萄糖酶 (BGL) 和性酸酶 (ALP) 在内的各种酶封装的TpAzo COFs的单水合成.
  • 固态2DNMR相关光谱检测分子水平的酶-COF相互作用.
  • 散射式扫描近场光学显微镜 (s-SNOM) 和纳米级里埃变换红外光谱 (nanoFTIR) 用于验证.
  • 用于测量酶活性,在变质条件下的稳定性 (SDS) 和可回收性.

主要成果:

  • 在TpAzo COF中成功封装多种酶和蛋白质.
  • 直接证实了酶与COF骨干之间的分子相互作用,证实了结构完整性.
  • 封装的BGL和ALP保持了高的催化活性,并且可回收使用长达10个循环.
  • 在1-15%的SDS溶液中,COF封装显著提高了BGL的稳定性,减轻了变性.

结论:

  • 为酶-COF复合物建立了一个强大的单水合成策略.
  • 酶-COF相互作用是提高酶稳定性和可重复使用性的关键.
  • 这种方法提供了一种可行的方法,用于制造稳定,可重复使用的生物催化剂.