骨肌肉,神经肌肉器官和组合物:一个范围审查
Jiang-Li Yang1, Shi-Yan Qian1, Min-Li Chen2
1Department of Neurology, The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, China.
EBioMedicine
|June 27, 2025
概括
骨肌肉有机体 (SMOs),神经肌肉有机体 (NMOs) 和组合体为研究神经肌肉结 (NMJ) 功能和像ALS这样的疾病提供先进的体外模型. 本综述综合了它们的发展,应用和未来研究的挑战.
科学领域:
- 生物医学工程 生物医学工程
- 发展生物学 发展生物学
- 神经科学是一个神经科学.
背景情况:
- 神经肌肉结 (NMJ) 功能障碍与诸如肌缩性侧面硬化症 (ALS) 等疾病有关.
- 诸如骨肌肉有机体 (SMOs),神经肌肉有机体 (NMOs) 和组合体等体外模型正在出现,用于研究NMJ.
- 范围审查对于绘制新兴研究领域和方法论至关重要.
研究的目的:
- 对用于肌肉功能建模的SMO,NMO和装配体的研究进行范围审查.
- 确定这一领域的关键概念,方法和应用.
- 为未来神经肌肉建模研究提供基础.
主要方法:
- 在五个数据库中搜索相关研究.
- 包括17项研究,重点关注SMO,NMO和组合体的开发和应用.
- 总结了分化协议,细胞组成和研究结果.
主要成果:
- 突出了用于肌肉功能建模的SMO,NMO和装配体的进步.
- 使用Wnt信号激应剂 (CHIR99021) 和bFGF的详细分化协议.
- 审查了包括运动神经元,神经干细胞,施万细胞和星体细胞在内的细胞组件.
结论:
- 中小型组织,NMO和组合体是体外神经肌肉研究的宝贵工具.
- 关键的挑战包括iPSC供体选择,标准化,血管化和成像.
- 本综述为推进神经肌肉建模技术奠定了基础.
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