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储存温度和时间对单次收获血小板丰富血中生长因子度的影响

Yulin Wang¹, Xiaona Fu¹, Dongdong Cao²

¹Department of Blood Transfusion, Qingdao Chengyang People's Hospital, No.600, Changcheng Road, Chengyang District, Qingdao, Shandong 266109, China.

Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis

|June 27, 2025

概括

储存富血小板血 (PRP) 影响必需生长因子度. 与室温或冷相比,冷藏 (4°C) 对PRP的治疗潜力提供了更好的稳定性.

科学领域:

再生医学是一种再生医学.
生物技术是生物技术.
细胞生物学细胞生物学

背景情况:

富血小板血 (PRP) 治疗由于其治疗潜力而引起了显著的兴趣.
优化PRP的储存条件对于其增加的临床应用和疗效至关重要.
了解储存温度和时间对PRP生物活性的影响至关重要.

研究的目的:

研究存储温度和时间如何影响富血小板血 (PRP) 中的关键生长因子度.
确定最佳的储存条件,以保持临床使用PRP的生物活性.

主要方法:

从10名健康的捐赠者中准备了PRP.
在室温 (22°C),4°C和-65°C下保存PRP样本.
在基线,第一天,第三天和第六天使用特定激活方法后的三明治免疫试验测量PDGF-AA,TGF-β1,VEGF和b-FGF度.

主要成果:

在所有储存条件下,血小板衍生生长因子-AA (PDGF-AA) 度随着时间的推移而下降.
转化生长因子-β (TGF-β1) 水平增加,在第6天达到峰值.
血管内皮生长因子 (VEGF) 和纤维细胞生长因子-基本 (b-FGF) 显示度降低,特别是在室温和-65°C,而4°C显示中等稳定性.

结论:

储存温度和时间显著影响PRP中基本生长因子的度.
与室温或冷相比,4°C的冷藏似乎为保护生长因子的完整性提供了更有利的平衡.
优化的储存协议对于保持PRP的生物活性特性和最大限度地提高其在临床应用中的治疗效益至关重要.

关键词:

增长因素是指增长的因素.血小板血小板血小板血小板血小板富含血小板血的血含量是多少再生医学是一种再生医学.

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