相关实验视频

Updated: Sep 17, 2025

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Molecular Analysis of Endothelial-mesenchymal Transition Induced by Transforming Growth Factor-β Signaling

Published on: August 3, 2018

可溶性α-Klotho保护皮肤微血管内皮细胞免受内皮细胞转移到介质细胞的伤害

Eloisa Romano¹, Irene Rosa², Eleonora Sgambati³

¹Department of Experimental and Clinical Medicine, Section of Internal Medicine, University of Florence, Largo Brambilla 3, Florence 50134, Italy.

|June 27, 2025

概括

可溶性α-Klotho (sKL) 激素通过抑制内皮细胞到介质细胞过渡 (EndMT) 来预防皮肤纤维化. 这项研究表明,sKL保护人类皮肤微血管内皮细胞免受增长因子β1诱导的EndMT的转化,从而保持内皮功能.

科学领域:

细胞生物学细胞生物学
皮肤病学皮肤病学
内分泌学在内分泌学.

背景情况:

皮肤纤维化由内皮细胞转移到介质酶 (EndMT) 驱动皮肤纤维化.
可溶性α-Klotho (sKL) 在皮肤纤维化和EndMT中的作用尚不清楚.

研究的目的:

研究sKL是否抑制转化生长因子β1 (TGFβ1) 诱导的EndMT在人类皮肤微血管内皮细胞 (H-dMVECs) 中.

主要方法:

用sKL预处理H-dMVEC,并用TGFβ1.1进行刺激.
评估了形态变化,内皮细胞/介质细胞标记物表达 (qPCR,西部斑点,免疫光) 和细胞收缩性 (原凝收缩试验).

主要成果:

TGFβ1诱导的EndMT,其特征是形态变化,内皮标记物丧失,中细胞标记物增加和收缩能力增强.
sKL预治疗显著减轻了这些TGFβ1诱导的变化,维持了内皮表型并减少了收缩活性.

结论:

sKL有效地防止H-dMVEC中TGFβ1诱导的EndMT.
sKL显示出作为皮肤纤维化治疗剂的潜力.

关键词:

皮肤纤维化皮肤纤维化内皮细胞到介质细胞的过渡.微血管内皮细胞微血管内皮细胞 α - 克洛托霍 (Klotho) 是一种

更多相关视频

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

TGF-β-mediated Endothelial to Mesenchymal Transition EndMT and the Functional Assessment of EndMT Effectors using CRISPR/Cas9 Gene Editing

Published on: February 26, 2021

Author Spotlight: Magnetic Bead-Based Isolation of Murine Dermal Lymphatic Endothelial Cells

Author Spotlight: Magnetic Bead-Based Isolation of Murine Dermal Lymphatic Endothelial Cells

Published on: July 21, 2023

相关概念视频

Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...